The Mitochondrial Genomes of Aquila fasciata and Buteo lagopus (Aves, Accipitriformes): Sequence, Structure and Phylogenetic Analyses.
The Mitochondrial Genomes of Aquila fasciata and Buteo lagopus (Aves, Accipitriformes): Sequence, Structure and Phylogenetic Analyses.
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Aquila fasciata和Buteo Lagopus（Aves，Accipitriformes）的线粒体基因组：序列，结构和系统发育分析。
DOI：
10.1371/journal.pone.0136297
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Kan X
中科院分区：
文献类型：
--
作者：
Jiang L;Chen J;Wang P;Ren Q;Yuan J;Qian C;Hua X;Guo Z;Zhang L;Yang J;Wang Y;Zhang Q;Ding H;Bi D;Zhang Z;Wang Q;Chen D;Kan X
鹰科是非雀形目鸟类中最大的类群之一，包括68属243种，分布于全球各地。在本研究中，我们确定了完整的线粒体序列的两个物种的accipitrid，即Aquila fasciata和Buteo lagopus，并进行了比较有丝分裂基因组分析整个家庭。A. fasciata和B. lagopus和lagopus分别为18，513和18，559 bp，A + T含量分别为54.2%和55.0%。两种鹰雀mtDNA H链编码的12个PCG均存在明显的正AT偏置和负GC偏置，而L链编码的MT-ND 6则存在明显的正AT偏置和负GC偏置。在A的MT-ND 3基因的174位多了一个核苷酸“C”。fasciata，而在B中未观察到。lagopus在结构域II的6个保守序列盒中，分别命名为盒F、E、D、C、CSBa和CSBb。fasciata和B. lagopus并对鹰科线粒体基因进化的速率和模式进行了估计。鹰科中MT-ATP 8基因的dN/dS最高（0.32493），MT-CO 1基因的dN/dS最低（0.01415）。有丝分裂分析支持了鹰形目的单系性，而Cathartidae是该目平衡的基础。此外，我们进行了系统发育分析，使用其他两个数据集（两个线粒体基因座，核和线粒体基因座组合）。我们的研究结果表明，Aquilinae亚科和目前所有的多型属该亚科是单系的。这两个新的线粒体DNA数据将有助于阐明鹰形目的系统发育关系和进化过程。
The family Accipitridae is one of the largest groups of non-passerine birds, including 68 genera and 243 species globally distributed. In the present study, we determined the complete mitochondrial sequences of two species of accipitrid, namely Aquila fasciata and Buteo lagopus, and conducted a comparative mitogenome analysis across the family. The mitogenome length of A. fasciata and B. lagopus are 18,513 and 18,559 bp with an A + T content of 54.2% and 55.0%, respectively. For both the two accipitrid birds mtDNAs, obvious positive AT-skew and negative GC-skew biases were detected for all 12 PCGs encoded by the H strand, whereas the reverse was found in MT-ND6 encoded by the L strand. One extra nucleotide‘C’is present at the position 174 of MT-ND3 gene of A. fasciata, which is not observed at that of B. lagopus. Six conserved sequence boxes in the Domain II, named boxes F, E, D, C, CSBa, and CSBb, respectively, were recognized in the CRs of A. fasciata and B. lagopus. Rates and patterns of mitochondrial gene evolution within Accipitridae were also estimated. The highest dN/dS was detected for the MT-ATP8 gene (0.32493) among Accipitridae, while the lowest for the MT-CO1 gene (0.01415). Mitophylogenetic analysis supported the robust monophyly of Accipitriformes, and Cathartidae was basal to the balance of the order. Moreover, we performed phylogenetic analyses using two other data sets (two mitochondrial loci, and combined nuclear and mitochondrial loci). Our results indicate that the subfamily Aquilinae and all currently polytypic genera of this subfamily are monophyletic. These two novel mtDNA data will be useful in refining the phylogenetic relationships and evolutionary processes of Accipitriformes.