Testicular hypertrophy in rats.
Testicular hypertrophy in rats.
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大鼠睾丸肥大。
DOI：
10.1530/jrf.0.0210537
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发表时间：
1970
期刊：
影响因子：
--
通讯作者：
M. D. Liang
中科院分区：
文献类型：
--
作者：
D. S. Liang;M. D. Liang
Lipschutz（1922）进行了睾丸肥大的实验，他指出对侧睾丸加速生长，迅速达到最大重量，但单侧睾丸切除术后没有发生真正的肥大。Grant（1955）指出，三只白化大鼠单侧睾丸切除术后睾丸体积增加。Shellabarger（1963）指出，切除单个睾丸后，睾丸重量没有增加。作为内分泌器官，剩余睾丸的间质细胞，包括间质细胞，应反映单侧去势的影响。由于精子发生是受激素控制的，精子小管可能会通过增加配子的产生来做出反应，就像单侧受精后单独的剩余卵巢一样。本工作旨在重新检验上述观察结果。实验用Sprague-Dawley大鼠150只，体重80 ~ 100 g。这些动物被分成三组。对照组（第一组）50只大鼠，称重饲养4周后处死，尸检重称。睾丸也被取出并称重。第二组50只大鼠行右侧单侧睾丸切除术。4周后将其杀死并称重。取下剩余睾丸，称重，固定在Zenker溶液中，按6 \g=m\厚度切片，并用血红素和伊红染色。第三组50只大鼠均行右侧单侧睾丸切除术。每周皮下注射2次，共注射睾酮10 mg，剂量0 ~ 05 ml。4周后处死这些大鼠并称重。剩余睾丸称重固定，切片并用苏木精和伊红染色。该数值由每只动物的睾丸重量除以体重计算（TW/BW见表1）。所有睾丸在最宽处与纵轴成直角切片，对小管和间质细胞进行完整的横切面计数。然后测量管径。1组大鼠术前平均体重为87 g， 2组为82 g， 3组为83 g，尸检时平均体重分别为236 g、212 g和200 g。1组大鼠左睾丸尸检时平均重量为1 ~ 30g， 2组剩余睾丸尸检时平均重量为1 ~ 27g。
Experiments on hypertrophy of the testis have been carried out by Lipschutz (1922) who stated there was accelerated growth of the contralateral testis which rapidly reached maximum weight, but that no true hypertrophy occurred after unilateral orchidectomy. Grant (1955) stated that an increase in testicular volume occurred after unilateral orchidectomy in three albino rats. Shellabarger (1963) stated there was no increase in testicular weight after removal of a single testis. Being an endocrine organ, the interstitial cells, including Leydig cells, of the remaining testis should reflect the effects of unilateral castration. Since spermatogenesis is hormonally controlled, the seminiferous tubule might be expected to respond by enhancing gamete production as do solitary remaining ovaries after unilateral spaying. The present work was designed to re-examine the above observations. One hundred and fifty Sprague-Dawley rats weighing 80 to 100 g were used in the experiments. The animals were divided into three groups. The control group (Group 1) consisted of fifty rats, which were weighed and kept for 4 weeks, and then killed and re-weighed at autopsy. The testes were also removed and weighed. Group 2 consisted of fifty rats, each ofwhich was subjected to right unilateral orchidectomy. These were killed 4 weeks later and weighed. The remaining testis was removed, weighed, fixed in Zenker's solution, sectioned at 6 \g=m\thickness, and stained with haematoxylin and eosin. Group 3 also consisted of fifty rats, each of which was subjected to right unilateral orchidectomy. Testosterone cypionate, 0-05 ml, was injected twice a week subcutaneously for a total of 10 mg testosterone weekly. These rats were then killed 4 weeks later and weighed. The remaining testes were weighed and fixed, and then sectioned and stained with haematoxylin and eosin. The value was calculated by testicular weight over body weight ofeach animal (TW/BW in Table 1). All the testes were sectioned at right angles to their longitudinal axes at the widest point and a complete cross-section count of both the tubules and interstitial cells was made. The tubular diameters were then measured. The average pre-operative body weight was 87 g for the rats in Group 1, 82 g for those in Group 2, and 83 g for those in Group 3, while the average weight at autopsy was 236 g, 212 g and 200 g, respectively. The average weight for the left testis of rats in Group 1 at autopsy was 1-30 g, while the average weight of the remaining testis in Group 2 was 1-27 g,