Protection against influenza virus infection by intranasal vaccine with surf clam microparticles (SMP) as an adjuvant
Protection against influenza virus infection by intranasal vaccine with surf clam microparticles (SMP) as an adjuvant
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DOI：
10.1002/jmv.20647
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发表时间：
2006-07-01
影响因子：
12.7
通讯作者：
Hasegawa, Hideki
中科院分区：
文献类型：
--
作者：
Ichinohe, Takeshi;Watanabe, Izumi;Hasegawa, Hideki
一种安全有效的佐剂是开发鼻内灭活流感疫苗增强粘膜免疫应答所必需的。本研究证明了来自天然海蛤的海蛤微粒（SMP）作为鼻内流感疫苗佐剂的有效性。在BALB/c小鼠中，当与灭活的A/PR 8（H1N1）流感病毒血凝素疫苗鼻内联合施用时，检查SMP的佐剂效应。接种SMP疫苗可诱导鼻洗液中的高抗PR 8血凝素（HA）特异性免疫球蛋白A（伊加）应答和血清中的免疫球蛋白G（IgG）应答，从而保护机体免受A/PR 8病毒引起的鼻限制性感染和致死性肺部感染。此外，SMP与A/Yamagata（H1N1）、A/Beijing（H1N1）或A/Guizhou（H3 N2）疫苗一起给药，在鼻感染模型中对A/PR 8病毒攻击提供了完全保护，表明SMP佐剂疫苗可提供针对变异流感病毒的交叉保护。提示SMP可作为一种新的安全有效的粘膜佐剂用于鼻内疫苗接种以对抗流感病毒感染。
A safe and effective adjuvant is necessary to enhance mucosal immune responses for the development of an inactivated intranasal influenza vaccine. The present study demonstrated the effectiveness of surf clam microparticles (SMP) derived from natural surf clams as an adjuvant for an intranasal influenza vaccine. The adjuvant effect of SMP was examined when co-administered intranasally with inactivated A/PR8 (H1N1) influenza virus hemagglutinin vaccine in BALB/c mice. Administration of the vaccine with SMP induced a high anti-PR8 haemagglutinin (HA)specific immunoglobulin A (IgA) response in the nasal wash and immunoglobulin G (IgG) response in the serum, resulting in protection against both nasal-restricted infection and lethal lung infection by A/PR8 virus. In addition, administration of SMP with A/Yamagata (H1N1), A/Beijing (H1N1), or A/Guizhou (H3N2) vaccine conferred complete protection against A/PR8 virus challenge in the nasal infection model, suggesting that SMP adjuvanted vaccine can confer cross-protection against variant influenza viruses. The use of SMP is suggested as a new safe and effective mucosal adjuvant for nasal vaccination against influenza virus infection.