CYTOKINES CAUSE FUNCTIONAL AND STRUCTURAL DAMAGE TO ISOLATED ISLETS OF LANGERHANS
CYTOKINES CAUSE FUNCTIONAL AND STRUCTURAL DAMAGE TO ISOLATED ISLETS OF LANGERHANS
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DOI：
10.1111/j.1398-9995.1985.tb02681.x
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发表时间：
1985-01-01
期刊：
影响因子：
12.4
通讯作者：
NERUP, J
中科院分区：
文献类型：
--
作者：
MANDRUPPOULSEN, T;BENDTZEN, K;NERUP, J
细胞因子是一种可溶性、抗原非特异性、非免疫球蛋白的介体，由参与细胞免疫反应的血液单个核细胞产生和分泌。为了验证细胞因子参与胰岛β细胞自身免疫破坏导致胰岛素依赖型糖尿病的可能性，将分离的人或大鼠胰岛与来自健康供者的富含细胞因子的无细胞单个核细胞上清液孵育7天，刺激或不刺激结核菌素或植物血凝素的纯化蛋白衍生物。葡萄糖刺激胰岛素释放，富含细胞因子的培养上清液孵育的胰岛胰岛素和胰升糖素含量显著降低。这种胰岛功能的损害是由于富含细胞因子的上清液的细胞毒性作用，通过正常的光学显微镜形态的解体来判断。
Cytokines are soluble, antigen non-specific, non-immunoglobulin mediators produced and secreted by blood mononuclear cells interacting in the cellular immune-response. To test the possibility that cytokines participate in the autoimmune destruction of the pancreatic beta-cells leading to insulin-dependent diaetes mellitus, isolated human or rat islets of Langerhans were incubated for 7 days with cytokine-rich, cell-free supernatants of blood mononuclear cells from healthy human donors stimulated with or without purified protein derivative of tuberculin or phytohaemagglutinin. Glucose stimulated insulin-release, and contents of insulin and glucagon in islets incubated with cytokine-rich supernatants were markedly reduced. This impairment of islet function was due to a cytotoxic effect of cytokine-rich supernatants as judged by disintegration of normal light-microscopic morphology.