A QTL for high grain yield under lowland drought in the background of popular rice variety Sabitri from Nepal
A QTL for high grain yield under lowland drought in the background of popular rice variety Sabitri from Nepal
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DOI：
10.1016/j.fcr.2013.01.019
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发表时间：
2013-03-20
影响因子：
5.8
通讯作者：
Kumar, Arvind
中科院分区：
文献类型：
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作者：
Yadaw, Ram Baran;Dixit, Shalabh;Kumar, Arvind
干旱是雨养稻区减产的主要原因。多地点试验中的高 GXE 相互作用是育种者开发在不同环境下具有稳定性能的育种品系的主要挑战。在由杂交 IR77298-5-6-18/2*Sabitri 开发的 BC1 衍生作图群体中，在靠近 RM231 的 3 号染色体短臂上鉴定出一个大效应 QTL qDTY(3.2)。该 QTL 解释了严重低地干旱条件下谷物产量表型变异的 23.4%，并且在 DS2011、WS2011 和 WS2012 的菲律宾和尼泊尔不同的水分胁迫七十年代中具有一致的影响。尽管该 QTL 与与开花时间相关的 HD9 基因座共定位，但在严重和中度干旱胁迫条件下，无论该群体的开花时间如何，该 QTL 都会对该 QTL 阳性品系的谷物产量产生影响。该基因座的基因内容分析显示存在多种与胁迫耐受性以及与开花时间相关的基因。该研究使用批量分离分析 (BSA) 并结合在多个地点进行筛选，以同时识别和测试 qDTY(3.2) 在目标环境中的效果。这些结果表明，管理性旱季筛选与目标位点绘图群体的表型分析以及 BSA 等基因分型方法相结合，可以鉴定和验证 QTL 对干旱条件下谷物产量的影响，从而通过对已识别的 QTL 进行标记辅助育种，快速开发流行品种的耐旱版本。 (C) 2013 Elsevier B.V. 保留所有权利。
Drought is the major cause of the yield reduction in rainfed rice areas. High GXE interaction in multi-location trials is a major challenge for breeders in developing breeding lines with a stable performance over diverse environments. In a BC1-derived mapping population developed from the cross IR77298-5-6-18/2*Sabitri, a large-effect QTL, qDTY(3.2), was identified on the short arm of chromosome 3 near RM231. This QTL explained 23.4% of the phenotypic variance for grain yield under severe lowland drought and had a consistent effect across varying water stress seventies in the Philippines and Nepal in DS2011, WS2011 and WS2012. Although this QTL co-localizes with the HD9 locus related to flowering time, an effect of this QTL was seen on the grain yield of QTL-positive lines under severe and moderate drought stress conditions regardless of the flowering time in this population. The gene content analysis of this locus showed the presence of a wide range of genes related to stress tolerance along with those related to flowering time. The study used bulk segregant analysis (BSA) coupled with screening at more than one location to simultaneously identify and test the effect of qDTY(3.2) in the target environment. These results indicate that managed dry-season screening coupled with phenotyping of mapping populations at the target site and genotyping approaches such as BSA allow the identification and validation of the effect of QTLs on grain yield under drought that will lead to the rapid development of drought-tolerant versions of popular varieties through marker-assisted breeding of the identified QTLs. (C) 2013 Elsevier B.V. All rights reserved.