Chitosan Microbeads as Supporter for Pseudomonas putida with Surface Displayed Laccases for Decolorization of Synthetic Dyes
Chitosan Microbeads as Supporter for Pseudomonas putida with Surface Displayed Laccases for Decolorization of Synthetic Dyes
复制标题
壳聚糖微珠作为恶臭假单胞菌的支持物，具有表面展示漆酶，用于合成染料的脱色
DOI：
10.3390/app9010138
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发表时间：
2019
影响因子：
2.7
通讯作者：
Li Lin
中科院分区：
文献类型：
--
作者：
Bai Zhiqiang;Sun Xiaowen;Yu Xun;Li Lin
被工业染料污染的各种未经处理的废水对自然环境构成严重污染危害，并对公众健康构成严重威胁。目前的研究报告了一种新的材料与配置壳聚糖基质和工程化的恶臭假单胞菌细胞与表面展示漆酶，可以脱色五个工业染料。通过自制装置制备了5种不同粒径的壳聚糖微球。在优化的固定化条件下，将恶臭假单胞菌固定化在CTS-MB上，形成降解-生物吸附双功能脱色复合物。扫描电子显微镜和红外分析证实了细胞成功地固定到CTS-MB基质上。表面接枝醛基的CTS-MB 1的优化（aCTS-MB 1）复合物对酸性绿色25和酸性红18的脱色率在pH 2.5-8.5和15-85 °C的较宽温度范围内均能达到94%以上;该络合物还能够有效地使直接红243、活性蓝220和活性蓝198脱色。此外，aCTS-MB 1复合物在连续和再生脱色反应中显示出良好的活性。因此，具有改善的机械强度和性能的壳聚糖固定化脱色材料显示出进一步大规模或连续工艺的潜力。
Various untreated wastewaters contaminated with industrial dyes pose significant pollution hazards to the natural environment as well as serious risks to public health. The current study reports a new material with a configurative chitosan matrix and engineered Pseudomonas putida cells with surface-displayed laccases that can decolorize five industrial dyes. Through a self-configuring device, five chitosan microbeads (CTS-MBs) with different particle sizes were prepared. P. putida cells were then immobilized onto the CTS-MBs under optimized immobilization conditions, forming a degrading-biosorbent dual-function decolorization complex. Scanning electron microscope and infrared analysis confirmed the successful immobilization of the cells onto the CTS-MB matrix. The optimized CTS-MB1 with surface-grafted aldehyde groups (aCTS-MB1) complex was capable of decolorizing Acid Green 25 and Acid Red 18 over a pH range of 2.5–8.5 and a relatively broad temperature range of 15–85 °C, with a maximum relative decolorization value of over 94%; the complex was also able to efficiently decolorize Direct Red 243, Reactive Blue 220 and Reactive Blue 198. Moreover, the aCTS-MB1 composite showed favorable activity in continuous and regenerative decolorization reactions. Therefore, the chitosan-immobilized decolorizing material, with both improved mechanical strength and performance, shows potential for further large-scale or continuous processes.