Deiminated Epstein-Barr virus nuclear antigen 1 is a target of anti-citrullinated protein antibodies in rheumatoid arthritis
Deiminated Epstein-Barr virus nuclear antigen 1 is a target of anti-citrullinated protein antibodies in rheumatoid arthritis
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DOI：
10.1002/art.21629
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发表时间：
2006-03-01
影响因子：
--
通讯作者：
Migliorini, P
中科院分区：
文献类型：
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作者：
Pratesi, F;Tommasi, C;Migliorini, P
客观的。为了检验以下假设：含有 Arg-Gly 重复序列的病毒序列的脱亚胺化可以产生可被类风湿性关节炎 (RA) 血清中存在的抗瓜氨酸蛋白抗体 (ACPA) 识别的表位。方法。合成了源自 Epstein-Barr 病毒 (EBV) 编码的 Epstein-Barr 核抗原 1 (EBNA-1) 的多种抗原肽，用瓜氨酸取代精氨酸，并用于筛选 RA 血清。通过亲和层析纯化抗环瓜氨酸肽抗体，并在一组体外脱亚胺蛋白上进行测试。使用 EBV 感染的细胞系，通过免疫沉淀评估它们结合体内脱亚胺化蛋白的能力。结果。在 50% 的 RA 血清和
Objective. To test the hypothesis that deimination of viral sequences containing Arg-Gly repeats could generate epitopes recognized by anti-citrullinated protein antibodies (ACPAs) that are present in rheumatoid arthritis (RA) sera.Methods. Multiple antigen peptides derived from Epstein-Barr virus (EBV)-encoded Epstein-Barr nuclear antigen 1 (EBNA-1), were synthesized, substituting the arginines with citrulline, and were used to screen RA sera. Anti-cyclic citrullinated peptide antibodies were purified by affinity chromatography and tested on a panel of in vitro deiminated proteins. Their ability to bind in vivo deiminated proteins was evaluated by immunoprecipitation, using EBV-infected cell lines.Results. Antibodies specific for a peptide corresponding to the EBNA-]([35-58) sequence containing citrulline in place of arginine (viral citrullinated peptide [VCP]) were detected in 50% of RA sera and in