Analysis of antigen-presenting functionality of cultured rat hepatic stellate cells and transdifferentiated myofibroblasts
Analysis of antigen-presenting functionality of cultured rat hepatic stellate cells and transdifferentiated myofibroblasts
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DOI：
10.1016/j.bbrc.2010.04.094
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发表时间：
2010-05-28
影响因子：
3.1
通讯作者：
Weiskirchen, Ralf
中科院分区：
文献类型：
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作者：
Bomble, Michael;Tacke, Frank;Weiskirchen, Ralf
在这里，我们证明，肝星状细胞（HSC）分离自刘易斯大鼠在体外抗原呈递细胞（APC）的功能，并能够处理和提出外源性抗原。我们显示激活的主要组织相容性复合体II（RT1BI）限制性T细胞杂交瘤特异性的豚鼠髓鞘碱性蛋白（gpMBP）与HSC共培养后。在HSC转分化为肌成纤维细胞（MFB）的过程中，APC功能显著降低，但可通过添加干扰素-γ（IFN-γ）而恢复。基于我们的研究结果，我们得出结论，HSC在肝脏免疫功能中起关键作用，IFN-γ治疗可能通过激活MFB中的APC功能介导其有益的治疗效果。(C)2010年爱思唯尔公司All rights reserved.
Here, we demonstrate that hepatic stellate cells (HSC) isolated from Lewis rats have in vitro antigen-presentation cell (APC) functionality and are able to process and present exogenous antigens. We show activation of a major histocompatibility complex II (RT1BI)-restricted T-cell hybridoma specific for guinea pig myelin basic protein (gpMBP) after coculture with HSC. During transdifferentiation of HSC into myofibroblasts (MFB) the APC function was markedly decreased but restorable by addition of interferon-gamma (IFN-gamma). Based on our findings we conclude that HSC play a key role in hepatic immune function and that IFN-gamma treatment might mediate its beneficial therapeutic effects via activation of APC function in MFB. (C) 2010 Elsevier Inc. All rights reserved.