Probing the interactions of phosphosulfomannans with angiogenic growth factors by surface plasmon resonance
Probing the interactions of phosphosulfomannans with angiogenic growth factors by surface plasmon resonance
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DOI：
10.1021/jm030180y
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发表时间：
2003-10-09
影响因子：
7.3
通讯作者：
Ferro, V
中科院分区：
文献类型：
--
作者：
Cochran, S;Li, CP;Ferro, V
在BIAcore 3000生物传感器上，通过表面等离子体共振（SPR）研究了磷酸磺基甘露聚糖抗癌剂PI-88（1）与血管生成生长因子FGF-1、FGF-2和VEGF的结合相互作用。与肝素相比，PI-88对FGF-1的亲和力至少高11倍，对VEGF的亲和力至少高11倍，但对FGF-2的亲和力至少低13倍。为了确定PI-88的结构特征，这是重要的生长因子结合，几个类似物，如去磷酸化PI-88和硫酸五糖，制备。这些类似物与FGF-1，FGF-2和VEGF的结合相互作用进行了类似的SPR研究，并确定了结构-活性关系。
The binding interactions of the phosphosulfomannan anticancer agent PI-88 (1) with the angiogenic growth factors FGF-1, FGF-2, and VEGF were studied by surface plasmon resonance (SPR) on a BIAcore 3000 biosensor. Compared with heparin, PI-88 has at least 11-fold higher affinity for FGF-1 and at least Mold higher affinity for VEGF, but at least 13-fold lower affinity for FGF-2. To define the structural features of PI-88 that are important for growth factor binding, several analogues, such as dephosphorylated PI-88 and a sulfated pentasaccharide, were prepared. The binding interactions of these analogues with FGF-1, FGF-2, and VEGF were similarly studied by SPR, and structure-activity relationships were determined.