The telobox, a Myb-related telomeric DNA binding motif found in proteins from yeast, plants and human
The telobox, a Myb-related telomeric DNA binding motif found in proteins from yeast, plants and human
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DOI：
10.1093/nar/24.7.1294
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发表时间：
1996-04-01
影响因子：
14.9
通讯作者：
Gilson, E
中科院分区：
文献类型：
--
作者：
Bilaud, T;Koering, CE;Gilson, E
酵母TTAGGG结合因子1(Tbf1)是通过在体外与脊椎动物端粒重复序列相互作用而被鉴定和克隆的。我们发现，位于其C末端的60个氨基酸的序列对DNA结合是关键的，该序列与Myb重复序列具有同源性，并且在五种植物蛋白质(其中两种已知与端粒相关序列结合)和两种人类蛋白质(包括端粒重复序列结合因子(TRF)和从一个未知蛋白质中预测的C末端多肽ORF2)中是保守的。我们证明了TRF111个C末端残基和ORF2残基能够与人的端粒重复序列结合，我们建议将这些蛋白质中发现的与Myb相关的特殊基序称为‘telobox’。针对Tbf1端盒的抗体在人类细胞系的核和有丝分裂染色体提取液中检测到两种蛋白质，而且这两种蛋白质在体外都与端粒重复序列特异结合。根据TRF和ORF2对端粒重复序列的高度亲和力，我们预测TRF和ORF2在人类端粒中发挥重要作用。
The yeast TTAGGG binding factor 1 (Tbf1) was identified and cloned through its ability to interact with vertebrate telomeric repeats in vitro. We show here that a sequence of 60 amino acids located in its C-terminus is critical for DNA binding, This sequence exhibits homologies with Myb repeats and is conserved among five proteins from plants, two of which are known to bind telomeric-related sequences, and two proteins from human, including the telomeric repeat binding factor (TRF) and the predicted C-terminal polypeptide, called orf2, from a yet unknown protein, We demonstrate that the 111 C-terminal residues of TRF and the 64 orf2 residues are able to bind the human telomeric repeats specifically, We propose to call the particular Myb-related motif found in these proteins the 'telobox'. Antibodies directed against the Tbf1 telobox detect two proteins in nuclear and mitotic chromosome extracts from human cell lines, Moreover, both proteins bind specifically to telomeric repeats in vitro. TRF is likely to correspond to one of them, Based on their high affinity for the telomeric repeat, we predict that TRF and orf2 play an important role at human telomeres.