Bioassay-guided isolation of anti-inflammatory and antinociceptive principles from a folk remedy, Rhododendron ponticum L. leaves
Bioassay-guided isolation of anti-inflammatory and antinociceptive principles from a folk remedy, Rhododendron ponticum L. leaves
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DOI：
10.1016/j.jep.2008.06.021
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发表时间：
2008-09-02
影响因子：
5.4
通讯作者：
Calis, Ihsan
中科院分区：
文献类型：
--
作者：
Erdemoglu, Nurgun;Akkol, Esra Kuepeli;Calis, Ihsan
人种药理学相关性：高山杜鹃（杜鹃花科）是用于治疗炎症性疾病和减轻风湿性疼痛和对土耳其传统medicine. Objective牙痛的研究：评价的抗炎和抗伤害作用的杜鹃花ponticum叶使用体内模型，并分离和化学表征的生物活性成分通过生物测定指导分馏程序。材料和方法：结果：与对照化合物相比，乙酸乙酯部位显示出明显的抗炎（28.4-40.7%抑制）和抗伤害（50.7%抑制）作用。通过生物活性测定指导的分级分离程序，从乙酸乙酯提取物中分离得到抗角叉菜胶引起的水肿和对苯醌引起的扭体的有效成分黄酮醇苷[金丝桃苷和异柠檬苷的混合物（1）和槲皮苷（2）]沿着一个黄烷酮苷[6-C-糖基柚皮素（3）]，并通过光谱技术鉴定了它们的结构。I和2也表现出显着的抗炎活性对12-0-tetradecanoyl-13-acetate（TPA）诱导的小鼠耳水肿model.Conclusion：本研究的结果支持利用土耳其民间医药的植物，并透露，黄酮类化合物是主要的抗炎和抗伤害性的原则，叶。(C)2008爱思唯尔爱尔兰有限公司保留所有权利。
Ethnopharmacological relevance: Rhododendron ponticum L (Ericaceae) is used for the treatment of inflammatory diseases and to alleviate rheumatic pain and against toothache in Turkish traditional medicine.Aim of the study: To evaluate the anti-inflammatory and antinociceptive effects of Rhododendron ponticum leaves using in vivo models, and isolation and chemical characterization of the biologically active constituents through bioassay-guided fractionation procedures.Material and Methods: Carrageenan-induced hind paw edema model was used for anti-inflammatory activity and p-benzoquinone induced abdominal contractions model for the antinociceptive activity assessment.Results: The ethylacetate fraction displayed marked anti-inflammatory (28.4-40.7% inhibition) and antinociceptive (50.7% inhibition) effects as compared to reference compounds. Through bioassay-guided fractionation and isolation procedures flavonol glycosides [a mixture of hyperoside and isocluercitrin (1) and quercitrin (2)] along with one flavanone glycoside [6-C-glycosylnaringenin (3)] were isolated as the active ingredients of ethylacetate extract against carrageenan-induced edema and p-benzoquinone-induced writhes and their structures were elucidated by spectral techniques. I and 2 also showed a significant anti-inflammatory activity against 12-0-tetradecanoyl-13-acetate (TPA)-induced mouse ear edema model.Conclusion: Results of the present study supported the utilization of the plant in Turkish folk medicine and revealed that flavones are the major anti-inflammatory and antinociceptive principles of the leaves. (C) 2008 Elsevier Ireland Ltd. All rights reserved.