Distinct expression of members of the LHT amino acid transporter family in flowers indicates specific roles in plant reproduction
Distinct expression of members of the LHT amino acid transporter family in flowers indicates specific roles in plant reproduction
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DOI：
10.1007/s00497-008-0074-z
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发表时间：
2008-06-01
影响因子：
--
通讯作者：
Tegeder, Mechthild
中科院分区：
文献类型：
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作者：
Foster, Justin;Lee, Yong-Hwa;Tegeder, Mechthild
植物的有性生殖需要花粉的适当发育，花粉萌发和通过雌蕊（花的雌性器官）的各种组织的管生长。最后，精细胞被释放出来，使雌配子体受精。这些过程需要所有相关组织的高代谢活动，并依赖于氮同化物的输送。然而，转运介导的氮通量大多是未知的。目前的工作提供了一个LHT氨基酸转运蛋白家族的成员在花粉发育和花粉雌蕊相互作用的表达分析。在花发育过程中分析拟南芥LHT的表达，并通过转运蛋白-GFP和启动子-GUS研究解决LHT功能的位置。洋葱细胞中的GFP-LHT定位表明，所有分析的LHT都靶向质膜。我们进一步表明，LHT在花药和雄配子体中表达，它们被认为在花粉发育和成熟的氨基酸运输中起作用。在萌发的花粉、花粉管和雌蕊的传递组织中的表达表明LHT在支持受精过程中的作用。总之，我们的研究表明，LHT功能在花中是细胞或组织特异性的，发育调节和高度协调的雄性和雌性组织之间。
Sexual plant reproduction necessitates proper development of pollen, pollen germination and tube growth through various tissues of the pistil, the female organ of the flower. Finally, sperm cells are released to fertilize the female gametophyte. These processes require high metabolic activities of all tissues involved and rely on the delivery of nitrogen assimilates for success. However, transporters mediating nitrogen fluxes are mostly unknown. The presented work provides an expression analysis of members of the LHT amino acid transporter family in relation to pollen development and pollen-pistil interaction. Expression of Arabidopsis LHTs was analyzed during flower development and the location of LHT function resolved by transporter-GFP and promoter-GUS studies. GFP-LHT localization in onion cells indicates that all LHTs analyzed are targeted to the plasma membrane. We further showed that LHTs are expressed in anthers and male gametophytes where they are proposed to function in transport of amino acids for pollen development and maturation. Expression in germinating pollen, pollen tubes and transmitting tissue of the pistil points to a role of LHTs in support of the fertilization process. Overall, our study suggests that LHT function in flowers is cell or tissue specific, developmentally regulated and highly coordinated between male and female tissue.