ADHESION BEHAVIOR OF THE RAT LYMPHOCYTE SUBPOPULATIONS (B-CELL AND T-CELL) ON THE SURFACE OF POLYSTYRENE POLYPEPTIDE GRAFT COPOLYMER
ADHESION BEHAVIOR OF THE RAT LYMPHOCYTE SUBPOPULATIONS (B-CELL AND T-CELL) ON THE SURFACE OF POLYSTYRENE POLYPEPTIDE GRAFT COPOLYMER
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DOI：
10.1002/jbm.820200104
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发表时间：
1986-01-01
期刊：
影响因子：
--
通讯作者：
SAKURAI, Y
中科院分区：
文献类型：
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作者：
MAEDA, M;KIMURA, M;SAKURAI, Y
为了开发特定的细胞分离器，新合成了表面具有微结构域结构的聚乙烯/多肽共聚物，并通过柱法检测了大鼠淋巴结淋巴细胞与这些材料的粘附行为。发现粘附于接枝共聚物的淋巴细胞的形态变化小于粘附于相应均聚物，即聚苯乙烯和聚(γ-L-谷氨酸苄酯)的细胞的形态变化。在没有血清蛋白的情况下检查淋巴细胞亚群（B 细胞和 T 细胞）的分离。发现 B 细胞的粘附选择性取决于微域结构，因为对于多肽含量为 50 wt% 的接枝共聚物观察到最高值 2,2。合成多肽衍生物可能是替代传统尼龙纤维和胎牛血清系统的有前途的材料。
Polyvinyl/polypeptide copolymers having microdomain structure on their surfaces were newly synthesized for the development of a specific cell separator, and the adhesion behavior of rat lymphnode lymphocytes with these materials was examined by column method. Morphologic change of lymphocytes adherent to the graft copolymers was found to be less than that of cells adherent to corresponding homopolymers, i.e., polystyrene and poly(.gamma.-benzyl L-glutamate). Separation of lymphocyte subpopulations (B cell and T cell) was examined in the absence of serum proteins. The adhesion selectivity for B cell was found to depend on the microdomain structure, since the highest value, 2,2, was observed for the graft copolymer with the polypeptide content of 50 wt%. Synthetic polypeptide derivatives may be promising materials which substitute for a conventional system with nylon fiber and fetal calf serum.