HUMAN LYMPHOCYTES-T POSSESS A LIMITED INVITRO LIFE-SPAN
HUMAN LYMPHOCYTES-T POSSESS A LIMITED INVITRO LIFE-SPAN
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DOI：
10.1016/0531-5565(89)90009-0
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发表时间：
1989-01-01
影响因子：
3.9
通讯作者：
EFFROS, RB
中科院分区：
文献类型：
--
作者：
PERILLO, NL;WALFORD, RL;EFFROS, RB
在衰老研究中，T淋巴细胞比其他可用的细胞类型具有一定的理论优势。免疫衰老是公认的哺乳动物衰老的一部分，并且可能与之直接相关，T淋巴细胞在功能、细胞表面抗原组成和其他因素方面具有很好的特征。然而，先前研究T细胞体外老化的努力受到倍增潜力可重复性差和发生特殊类型危机的阻碍。我们已经改善了正常人类T淋巴细胞长期体外繁殖的培养条件，从而消除了先前描述的相同操作培养和危机时期之间的差异。对109个个体培养物生长模式的分析显示，增殖寿命有限，累积种群数量加倍，与成人成纤维细胞的数量相对应。两种截然不同的细胞类型的体外寿命之间的这种一致性进一步支持了海弗利克极限作为一般生物学现象的概念。
The T lymphocyte offers certain theoretical advantages over other available cell types for the study of aging. Immunosenescence is a well-established part of, and may be directly relevant to, mammalian aging, and the T lymphocyte is well-characterized as to function, cell-surface antigen make-up, and other factors. However, prior efforts at studying in vitro aging of T cells have been hampered by poor reproducibility in doubling potential and the occurence of a peculiar type of crisis. We have improved the culture conditions for long-term in vitro propagation of normal human T lymphocytes so that previously described variability betwen identically manipulated cultures and the crisis period have been elminated. Analysis of the growth patterns of 109 individual cultures revealed a limited proliferative life span, with the number of cumulative population doublings corresponding to that reported for adult human fibroblasts. This accord between the in vitro life spans of two vastly different cell types lends further support to the concept of the Hayflick Limit as a general biological phenomenon.