CHEMICAL CONSTITUENTS OF Stellaria dichotoma var. lanceolata AND THEIR ANTI-INFLAMMATORY EFFECT ON LIPOPOLYSACCHARIDE-STIMULATED RAW 264.7 CELLS
CHEMICAL CONSTITUENTS OF Stellaria dichotoma var. lanceolata AND THEIR ANTI-INFLAMMATORY EFFECT ON LIPOPOLYSACCHARIDE-STIMULATED RAW 264.7 CELLS
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DOI：
10.1007/s10600-021-03306-0
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发表时间：
2021-01-26
影响因子：
0.8
通讯作者：
Fu, Xueyan
中科院分区：
文献类型：
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作者：
Dong, Lin;Zhou, Xirong;Fu, Xueyan
繁缕繁缕（Radix Stellariae）属于繁缕属，主要分布于中国宁夏及周边省份。该植物（繁缕）的根部作为中药（银柴胡）用于治疗热病后期的虚热[1]。先前的植物化学研究报道，已从这种植物的根中分离出一系列β-咔啉[1, 2]、环肽[3-5]、新木脂素和苯丙素[6]。它们具有抗炎 [7]、抗过敏 [6] 和血管扩张作用 [5]。在这里，我们描述了 20 种化合物 (1-20) 的分离和结构鉴定，并评估了 11 种繁缕化合物的抗炎作用。脂多糖 (LPS) 刺激的 RAW 264.7 细胞上的披针形 [8]。从繁缕的 70% 乙醇提取物中，获得了 20 种化合物 (1–20)。它们的结构被表征为邻苯二甲酸二丁酯(1)、stellarine B(2)、反式-2-甲基己-3-烯(3)、Z-十八烷基咖啡酸酯(4)、二克托胺I(5)、邻苯二甲酸双(2-乙基己基)酯(6)、N-tettratriacont-20, 23-二烯酸(7)、22-二氢软骨甾醇(8)、邻苯二甲酸异二丁酯 (9)、邻苯二甲酸 2-甲基丁基丙酯 (10)、吉来宝林 C (11)、二克托胺 XIV (12)、9, 10-双 (2-乙基己氧基羰基)-N-(1-己基庚基)-苝-3, 4-二甲酰亚胺 (13)、异香草酸 (14)、双(2-甲基庚基)邻苯二甲酸酯 (15)、次黄嘌呤-9-β-D-呋喃核苷 (16)、marinacarboline D (17)、1-甲基-α-D-葡萄糖 (18)、薄荷内酯 (19) 和二克托胺 XII (20)。所有成分，化合物 3、4、6-8、10、11、13、14、16 和 18 均为首次从繁缕属中分离得到。
Stellaria dichotoma var. lanceolata Bunge (Radix Stellariae), distributed mainly in Ningxia and neighboring provinces of China, belongs to the genus Stellaria L. The roots of this plant (Stellariae Radix) are used as traditional Chinese medicine (Yin-Chai-Hu) for the treatment of asthenic fever in the late stage of febrile diseases [1]. Previous phytochemical investigations reported that a series of β-carbolines [1, 2], cyclic peptides [3–5], neolignans, and phenylpropanoids [6] have been isolated from the roots of this plant. They show anti-inflammatory [7], anti-allergic [6], and blood vessel dilated effects [5]. Here, we describe the isolation and structural identification of 20 compounds (1–20) and evaluate the anti-inflammatory effects of 11 compounds from Stellaria dichotoma var. lanceolata on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells [8]. From a 70% ethanol extract of Radix Stellariae, 20 compounds (1–20) were obtained. Their structures were characterized as dibutyl phthalate (1), stellarine B (2), trans-2-methylhex-3-ene (3), Z-octadecyl caffeate (4), dichotomide I (5), bis (2-ethylhexyl) phthalate (6), N-tetratriacont-20, 23-dienoic acid (7), 22-dihydrochondrillasterol (8), phthalic acid isodibutyl ester (9), 2-methyl butyl propyl phthalate (10), geleboline C (11), dichotomide XIV (12), 9, 10-bis (2-ethylhexyloxycarbonyl)-N-(1-hexylheptyl)-perylene-3, 4-dicarboximide (13), isovanillic acid (14), bis (2-methylheptyl) phthalate (15), hypoxanthine-9-β-D-ribofuranoside (16), marinacarboline D (17), 1-methyl-α-D-glucose (18), menthalactone (19), and dichotomide XII (20). All the constituents, compounds 3, 4, 6–8, 10, 11, 13, 14, 16, and 18, were isolated for the first time from the genus Stellaria L.