Rhodopseudomonas acidophila strain 10050 contains photosynthetic LH2 antenna complexes that are not enriched with phosphatidylglycerol, and the phospholipids have a fatty acyl composition that is unusual for purple non-sulfur bacteria.
Rhodopseudomonas acidophila strain 10050 contains photosynthetic LH2 antenna complexes that are not enriched with phosphatidylglycerol, and the phospholipids have a fatty acyl composition that is unusual for purple non-sulfur bacteria.
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嗜酸红假单胞菌菌株 10050 含有不富含磷脂酰甘油的光合 LH2 天线复合物，并且磷脂具有紫色非硫细菌所不常见的脂肪酰基成分。
DOI：
10.1016/s0005-2728(02)00369-9
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
R. Cogdell
中科院分区：
文献类型：
--
作者：
N. Russell;J. Coleman;T. Howard;Evelyn Johnston;R. Cogdell
测定了嗜酸红球藻10050菌株在有氧和厌氧光照条件下的磷脂组成。有氧细胞中存在的主要磷脂是磷脂酰乙醇胺（PE; 54%）、磷脂酰甘油（PG; 24%）和心磷脂（二磷脂酰甘油，DPG）（14%），以及磷脂酰胆碱（PC; 5%）。当细胞在光照下进行无氧光合生长时，PE仍然是主要的磷脂（37-49%），但PC的比例发生了重大变化，增加到31- 33%，PG的含量相应减少到11-16%，DPG减少到4- 5%。与其他紫色非硫光合细菌相比，磷脂的脂肪酸组成是不寻常的，因为它含有16：0（29%），17：1（20%）和19：1（9%）加上几个主要的不饱和2-OH脂肪酸（9%）作为主要成分，当在黑暗中有氧生长时。相反，当在厌氧条件下光合生长时，存在<2%的17：1或19：1，而16：1和18：1的量增加，而16：0减少。纯化的捕光复合物2（LH 2）复合物的磷脂组成为PE（43%）、PC（42%）和DPG（15%）。出乎意料的是，没有PG与纯化的LH 2结合。这些发现与先前对其他几种光合细菌的研究形成对比，这些研究表明在光合生长时PG增加[Biochem.J.181（1979）339]。先前的假设，磷脂酰甘油有一些特定的作用，发挥捕光复合物的功能不能为真的RPS。嗜酸菌有人建议，特定的整合膜蛋白可能会强烈影响磷脂含量的主机膜，它们被插入。
The phospholipid composition of Rhodopseudomonas acidophila strain 10050 grown aerobically or anaerobically in the light was determined. The major phospholipids present in the aerobic cells were phosphatidylethanolamine (PE; 54%), phosphatidylglycerol (PG; 24%) and cardiolipin (diphosphatidylglycerol, DPG) (14%), together with phosphatidylcholine (PC; 5%). On moving the cells to anaerobic photosynthetic growth in the light PE remained the major phospholipid (37–49%), but there was a major change in the proportion of PC, which increased to 31–33%, and corresponding reductions in the contents of PG to 11–16% and DPG to 4–5%. The fatty acid composition of the phospholipids was unusual, compared with other purple non-sulfur photosynthetic bacteria, in that it contained 16:0 (29%), 17:1 (20%) and 19:1 (9%) plus several mainly unsaturated 2-OH fatty acids (9% total) as major components, when grown aerobically in the dark. In contrast when grown photosynthetically under anaerobic conditions there was <2% 17:1 or 19:1 present, while the amounts of 16:1 and 18:1 increased, and 16:0 decreased. The phospholipid composition of the purified light-harvesting complex 2 (LH2) complex was PE (43%), PC (42%) and DPG (15%). Unexpectedly, there was no PG associated with the purified LH2. These findings contrast with previous studies on several other photosynthetic bacteria, which had shown an increase in PG upon photosynthetic growth [Biochem. J. 181 (1979) 339]. The prior hypothesis that phosphatidyglycerol has some specific role to play in the function of light-harvesting complexes cannot be true for Rps. acidophila. It is suggested that specific integral membrane proteins may strongly influence the phospholipid content of the host membranes into which they are inserted.
影响因子：
5.7
作者：
Koepke, J;Hu, XC;Michel, H
通讯作者：
Michel, H