A rapid simple in situ hybridization method for herpes simplex virus employing a synthetic biotin‐labeled oligonucleotide probe: A comparison with immunohistochemical methods for HSV detection
A rapid simple in situ hybridization method for herpes simplex virus employing a synthetic biotin‐labeled oligonucleotide probe: A comparison with immunohistochemical methods for HSV detection
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采用合成生物素标记寡核苷酸探针的单纯疱疹病毒快速简单原位杂交方法：与 HSV 检测免疫组织化学方法的比较
DOI：
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发表时间：
1994
期刊：
影响因子：
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通讯作者：
K. Montone
中科院分区：
文献类型：
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作者：
John Y. Wang;K. Montone
我们利用人工毛细管作用技术（微探针染色系统）原位杂交（ISH）和从HSV糖蛋白C区合成的21碱基多生物素化寡核苷酸探针，检测了28例有疱疹病毒感染组织学证据的石蜡包埋组织标本。结果与检测HSV蛋白的快速简单免疫组织化学（IHC）方案进行了比较。除一个水痘带状疱疹病毒通过直接荧光抗体研究呈阳性外，其余标本均通过ISH和IHC检测到HSV。杂交信号均局限于细胞核内。在早期和晚期细胞病变作用中发现染色。免疫组化在感染细胞中产生强烈的核和/或细胞质信号，并在坏死区域染色，否则会被ISH保留。在3/5的标本中，免疫组化和/或ISH检测到HSV，组织学提示，但不能诊断为HSV感染。这两种技术对单纯疱疹病毒都是敏感和特异性的，可以在常规处理的组织中快速检测到病原体，并且可能在对单纯疱疹病毒感染的组织学印象不明确的情况下有用。单纯疱疹病毒的ISH可能是首选，因为它可以识别早期的单纯疱疹病毒感染，进而可以用抗病毒药物治疗。©1994 Wiley‐Liss, Inc。
We examined 28 paraffin‐embedded tissue specimens with histologic evidence of herpes virus infection by in situ hybridization (ISH) utilizing manual capillary action technology (Micro Probe Staining System) and a 21 base synthetic multibiotinylated oligonucleotide probe from the HSV glycoprotein C region. The results were compared to a rapid simple immunohistochemical (IHC) protocol for detection of HSV proteins. HSV was detected by ISH and IHC in all but one specimen which was shown to be positive for varicella zoster virus by direct fluorescent antibody studies. Hybridization signal was confined to the nucleus in all cases. Staining was identified in cells with early as well as late cytopathic effect. IHC produced intense nuclear and/or cytoplasmic signal in infected cells and stained in areas of necrosis which were otherwise spared by ISH. HSV was detected by IHC and/or ISH in 3/5 specimens with histology suggestive of, but not diagnostic for, HSV infection. Both techniques were sensitive and specific for HSV, resulted in rapid detection of the pathogen in routinely processed tissues, and may be useful in cases where the histologic impression is equivocal for HSV infection. ISH for HSV may be preferred because it can identify early HSV infection, which in turn can be treated with antiviral agents. © 1994 Wiley‐Liss, Inc.
疱疹病毒在其生命周期的裂解阶段的 DNA 复制。
DOI：
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发表时间：
1990
期刊：
Molecular biology & medicine
影响因子：
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作者：
Hammerschmidt,W;Sugden,B
通讯作者：
Sugden,B