An innovative nanoprobe for magnetic immunoassay: Individual γ-Fe2O3 nanoparticles; towards high sensitive and multiparametric detection
An innovative nanoprobe for magnetic immunoassay: Individual γ-Fe2O3 nanoparticles; towards high sensitive and multiparametric detection
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DOI：
10.1016/j.irbm.2011.09.004
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发表时间：
2011-11-01
期刊：
影响因子：
4.8
通讯作者：
Motte, L.
中科院分区：
文献类型：
--
作者：
de Montferrand, C.;Milosevic, I.;Motte, L.
利用单个超顺磁性γ - fe2o3纳米颗粒设计了一种新型的磁性免疫分析纳米标签。这个创新的候选人在这里面临着常用的微米珠子。创新的MIAplex (R)技术允许表征和量化两种类型的标签，具有竞争性的检测限制。在本文中，我们证明了用MIAplex (R)技术进行多参数免疫测试的可行性。外颗粒表面的羧基功能作为生物识别分子（如抗体、核酸）共价偶联的前体基团。(C) 2011 Elsevier Masson SAS。版权所有。
A new type of nanolabels for magnetic immunoassays was designed using individual superparamagnetic gamma-Fe2O3 nanoparticles. This innovative candidate is confronted here with commonly used micrometric beads. The innovative MIAplex (R) technology allows to characterize and to quantify both types of labels with a competitive limit of detection. In this paper, we demonstrate the feasibility of multiparametric immunotests with the MIAplex (R) technology. The carboxylic functions at the outer particle surface act as a precursor group for the covalent coupling of biological recognition molecules, such as antibodies, nucleic acids. (C) 2011 Elsevier Masson SAS. All rights reserved.