Hormone influence on the spatial regulation of IRT1 expression in iron-deficient Arabidopsis thaliana roots
Hormone influence on the spatial regulation of IRT1 expression in iron-deficient Arabidopsis thaliana roots
复制标题
DOI：
10.4161/psb.28787
复制
发表时间：
2014-01-01
影响因子：
2.9
通讯作者：
Ivanov, Rumen
中科院分区：
文献类型：
--
作者：
Blum, Ailisa;Brumbarova, Tzvetina;Ivanov, Rumen
IRON-REGULAT艾德TRAN SPORT ER 1（IRT 1）是拟南芥中土壤铁的主要输入者。它具有复杂的细胞内运输行为，包括质膜和内体之间的连续循环。内体定位的IRT 1的再循环需要分选NEXIN 1。在缺乏IRT 1的情况下，IRT 1被错误地定位为降解，导致植物铁吸收效率降低。因此，IRT 1启动子活性被限制在根的特定部分。我们测试了两种已知对IRT 1空间调节铁吸收有积极影响的激素的影响。我们发现，在野生型背景下的乙烯处理模拟了SNX功能丧失情况的影响。相反，生长素分裂IRT 1表达区，并迫使其向根的两端移动。这表明，IRT 1的表达沿着根调节乙烯-生长素的相互作用。
The IRON-REGULAT ED TRAN SPORT ER1 (IRT1) is the principal importer of soil iron in Arabidopsis thaliana. It has a complex intracellular trafficking behavior, including continuous cycling between plasma membrane and endosomes. SORTING NEXIN 1 is required for the recycling of endosome-localized IRT1. In its absence, IRT1 is mistargeted for degradation, resulting in reduced plant iron-uptake efficiency. Consequently, IRT1 promoter activity gets limited to a specific portion of the root. We tested the influence of two hormones known to positively affect iron uptake on IRT1 spatial regulation. We found that ethylene treatment in wild-type background mimics the effects of the SNX-loss-offunction situation. Conversely, auxin splits the IRT1 expression zone and forces it toward the two extremities of the root. This shows that IRT1 expression along the root is modulated by ethylene-auxin interplay.