Feasibility of measuring blood glucose concentration by near-infrared Raman spectroscopy.
Feasibility of measuring blood glucose concentration by near-infrared Raman spectroscopy.
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DOI：
10.1016/s1386-1425(96)01779-9
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发表时间：
1997-02
期刊：
影响因子：
--
通讯作者：
A. Berger;I. Itzkan;M. Feld
中科院分区：
文献类型：
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作者：
A. Berger;I. Itzkan;M. Feld
本文报道了用近红外拉曼光谱法测定人全血中葡萄糖浓度的方法。具有高于生理水平的葡萄糖的血液样品的拉曼光谱经由光纤通过比色皿的壁获得5分钟。偏最小二乘分析用于预测样品中的葡萄糖浓度。实现了3.6 mM葡萄糖的均方根预测误差，参考值和预测值之间的相关系数为0.99。这一结果是评估近红外拉曼光谱法进行临床准确血糖测量潜力的第一步。该技术能够测量其他拉曼活性血液成分的浓度;例如，还测量了碳酸氢盐。该方法最终可用于直接测量组织分析物。
We report the determinations of glucose concentration in human whole blood samples made using near-infrared Raman spectroscopy. Raman spectra of blood samples with above-physiological levels of glucose were acquired for 5 min through the wall of a cuvette via fiber optics. Partial least squares analysis was used to predict glucose concentrations in the samples. A root mean squared prediction error of 3.6 mM glucose was achieved with a correlation coefficient of 0.99 between reference and predicted values. This result is the first step in evaluating the potential of near-infrared Raman spectroscopy to perform blood glucose measurements with clinical accuracy. The technique is capable of measuring the concentration of other Raman-active blood constituents; as an example, bicarbonate was also measured. The method could eventually be useful for direct measurement of tissue analytes.