A case of variant hemoglobin (Hb Agenogi) with type 2 diabetes mellitus showed high HbA1c levels measured by immunoassay due to enhanced antigenicity
A case of variant hemoglobin (Hb Agenogi) with type 2 diabetes mellitus showed high HbA1c levels measured by immunoassay due to enhanced antigenicity
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一例患有 2 型糖尿病的变异血红蛋白 (Hb Agenogi) 病例由于抗原性增强，通过免疫测定测量显示 HbA1c 水平较高
DOI：
10.1007/s13340-018-0383-x
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发表时间：
2018
影响因子：
2.2
通讯作者：
M. Koga
中科院分区：
文献类型：
--
作者：
Y. Yamane;M. Tsumori;M. Ishibashi;M. Koga
HbA 1c是一种广泛用于糖尿病管理的生物标志物。变异血红蛋白的存在可能会干扰使用不同方法测量HbA 1c。本文描述了2型糖尿病患者HbA 1c（β 90 Glu → Lys）的免疫测定（IA）结果显示假性高水平，而标准模式（SM）下高效液相色谱（HPLC）结果显示假性低水平。为了阐明IA-HbA 1c水平假性升高的原因，通过各种方法测量HbA 1c。糖化白蛋白略高于参考范围。在这种情况下，通过HPLC在变体模式、亲和试验和酶法测定的HbA 1c显示了范围（平均值± 2SD：6.0-6.8%）。然而，通过几种HPLC-SM测量的HbA 1c显示假性低水平（4.1-4.4%）。IA-HbA 1c使用Fujirebio Inc.显示假性高水平（7.3-8.2%）;而使用Roche Ltd.生产的抗体的IA-HbA 1c显示预期范围（6.2-6.5%）。在Hb HbA 1 ogi的情况下，使用Fujirebio生产的抗体进行IA-HbA 1c检测时，结果出现假性高水平。β-珠蛋白链第90位密码子的突变可能增强N-末端肽区的抗原性，因此导致IA-HbA 1c中HbA 1c水平假性升高。
HbA1c is a widely utilized biomarker for the management of diabetes mellitus. The presence of variant hemoglobins might interfere with HbA1c measurement using different methods. We herein describe Hb Agenogi (β90Glu → Lys) with type 2 diabetes mellitus whose HbA1c measured by immunoassay (IA) showed falsely high levels while HbA1c measured by high-performance liquid chromatography (HPLC) in the standard mode (SM) showed falsely low levels. To clarify the cause of the falsely high-IA-HbA1c levels, HbA1c was measured by various methods. Glycated albumin was slightly higher than the reference range. HbA1c measured by HPLC in the variant mode, affinity assay and enzymatic assay showed the range (mean ± 2SD: 6.0–6.8%) in this case. However, HbA1c measured by several HPLC-SMs showed falsely low levels (4.1–4.4%). IA-HbA1c using an antibody manufactured by Fujirebio Inc. showed falsely high levels (7.3–8.2%); whereas, IA-HbA1c using an antibody manufactured by Roche Ltd. showed the expected range (6.2–6.5%). In the case of Hb Agenogi, IA-HbA1c using an antibody manufactured by Fujirebio yielded falsely high levels. The mutation at codon 90 of the β-globin chain might enhance antigenicity of the N-terminal peptide region and, therefore, lead to falsely high-HbA1c levels in IA-HbA1c.
影响因子：
3.2
作者：
Committee of the Japan Diabetes Society on the Diagnostic Criteria of Diabetes Mellitus;Seino Y;Nanjo K;Tajima N;Kadowaki T;Kashiwagi A;Araki E;Ito C;Inagaki N;Iwamoto Y;Kasuga M;Hanafusa T;Haneda M;Ueki K
通讯作者：
Ueki K