Effects of salts on activity of halophilic cellulase with glucomannanase activity isolated from alkaliphilic and halophilic Bacillus sp. BG-CS10
Effects of salts on activity of halophilic cellulase with glucomannanase activity isolated from alkaliphilic and halophilic Bacillus sp. BG-CS10
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盐对从嗜碱和嗜盐芽孢杆菌中分离的具有葡甘露聚糖酶活性的嗜盐纤维素酶活性的影响。
DOI：
10.1007/s00792-011-0403-2
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发表时间：
2011
期刊：
影响因子：
2.9
通讯作者：
Yanhe Ma
中科院分区：
文献类型：
--
作者：
Shunyi Li;Yanfen Xue;Liangwen Mao;Yanhe Ma
从西藏扎布耶盐湖中分离到一株嗜碱嗜盐芽孢杆菌BG-CS 10。从BG-CS10的基因组文库中鉴定出编码嗜盐纤维素酶的基因celB。CelB属于纤维素酶超家族和DUF 291超家族，其功能结构域未知，与GenBank中其他纤维素酶的同源性低于58%。纯化的重组蛋白（分子量：62 kDa）可水解含有β-1，4-键的可溶性纤维素底物，如羧甲基纤维素和葡甘露聚糖，但不具有外切葡聚糖酶和β-葡萄糖苷酶活性。因此，CelB是具有内切作用模式和葡甘露聚糖酶活性的纤维素酶。有趣的是，用2.5 M NaCl或3 M KCl使酶活性增加约10倍。此外，最佳温度分别为55°C和2.5 M NaCl，以及35°C无NaCl。这表明NaCl可以提高酶的热稳定性。CelB的K（m）和k（cat）值对于具有2.5 M NaCl的CMC为3.18 mg mL（-1）和26 s（-1），而不具有NaCl的CelB的K（m）和k（cat）值为6.6 mg mL（-1）和2.1 s（-1）。因此，该纤维素酶具有耐温、耐盐和耐pH的特点，是一种具有工业应用前景的纤维素酶，并为研究盐对蛋白质结构的影响提供了一种新的模型。
Alkaliphilic and halophilic Bacillus sp. BG-CS10 was isolated from Zabuye Salt Lake, Tibet. The gene celB, encoding a halophilic cellulase was identified from the genomic library of BG-CS10. CelB belongs to the cellulase superfamily and DUF291 superfamily, with an unknown function domain and less than 58% identity to other cellulases in GenBank. The purified recombinant protein (molecular weight: 62 kDa) can hydrolyze soluble cellulose substrates containing beta-1,4-linkages, such as carboxylmethyl cellulose and konjac glucomannan, but has no exoglucanase and β-glucosidase activities. Thus, CelB is a cellulase with an endo mode of action and glucomannanase activity. Interestingly, the enzyme activity was increased approximately tenfold with 2.5 M NaCl or 3 M KCl. Furthermore, the optimal temperatures were 55°C with 2.5 M NaCl and 35°C without NaCl, respectively. This indicates that NaCl can improve enzyme thermostability. The K ( m ) and k (cat) values of CelB for CMC with 2.5 M NaCl were 3.18 mg mL(-1) and 26 s(-1), while the K ( m ) and k (cat) values of CelB without NaCl were 6.6 mg mL(-1) and 2.1 s(-1). Thus, this thermo-stable, salt and pH-tolerant cellulase is a promising candidate for industrial applications, and provides a new model to study salt effects on the structure of protein.
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影响因子：
3.2
作者：
Christian K. Hansen;N. R. A. Meinke;Gilkes;D. Kilburn;R. C. Miller
通讯作者：
Christian K. Hansen;N. R. A. Meinke;Gilkes;D. Kilburn;R. C. Miller
影响因子：
1.8
作者：
Zhang, Gui-Min;Hu, Yong;Zhang, Xian-En
通讯作者：
Zhang, Xian-En
DOI：
10.1002/9780470123027.ch3
发表时间：
1984
期刊：
Advances in enzymology and related areas of molecular biology
影响因子：
--
作者：
P. Dey;E. Campillo
通讯作者：
P. Dey;E. Campillo
影响因子：
3.4
作者：
Chung‐Yi Wang;Yi-ru Hsieh;Chang-Chai Ng;H. Chan;Hsin-Tang Lin;W. Tzeng;Y. Shyu
通讯作者：
Chung‐Yi Wang;Yi-ru Hsieh;Chang-Chai Ng;H. Chan;Hsin-Tang Lin;W. Tzeng;Y. Shyu
影响因子：
3.8
作者：
Mevarech, M;Frolow, F;Gloss, LM
通讯作者：
Gloss, LM