Gastric Wash-Based Molecular Testing for Antibiotic Resistance in Helicobacter pylori
Gastric Wash-Based Molecular Testing for Antibiotic Resistance in Helicobacter pylori
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DOI：
10.1159/000332570
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发表时间：
2011-01-01
期刊：
影响因子：
3.2
通讯作者：
Itoh, Fumio
中科院分区：
文献类型：
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作者：
Baba, Satoshi;Oishi, Yoshichika;Itoh, Fumio
背景：已经开发出许多非侵入性测试来确定幽门螺杆菌感染的存在。然而，到目前为止，这些测试只能检测到它的存在。越来越多的抗生素耐药性幽门螺杆菌感染已被报道，并且已知它们与 23S rRNA 单核苷酸多态性 (SNP) 相关。我们假设，对从胃洗液中回收的幽门螺杆菌进行基因组分析不仅可以减少侵入性，而且还可以用作筛查测试和评估根除治疗的结果。方法：收集100例患者内镜检查时的活检标本和胃洗液。然后我们使用 PCR 和高通量焦磷酸测序分析了 23S rRNA、ureA 和 cagA 基因。结果：45% (44/97) 的患者尿A 检测呈阳性，42.3% (41/97) 的患者通过快速尿素酶检测呈阳性。通过这两种方法检测呈阳性的患者中，100% (35/35) 被观察到具有 cagA 基因。在这 35 名患者中，34.3% (12/35) 存在 23S rRNA SNP。结论：基于胃洗的 PCR 和焦磷酸测序测定可用于快速检测和估计临床分离的幽门螺杆菌中 23S rRNA SNP 的数量。这不仅是一种侵入性较小的技术，而且还可以诊断耐药性。版权所有 (C) 2011 S. Karger AG，巴塞尔
Background: A number of noninvasive tests have been developed to establish the presence of Helicobacter pylori infection. However, thus far these tests have only been capable of detecting its presence. An increasing number of antibiotic-resistant H. pylori infections have been reported and they are known to be correlated with 23S rRNA single nucleotide polymorphisms (SNPs). We hypothesized that genomic analysis of H. pylori recovered from gastric washes could not only be less invasive, but also useful as a screening test and for assessing the outcome of eradication therapy. Methods: Biopsy specimens and gastric washes were collected from 100 patients during endoscopic examination. Then we analyzed 23S rRNA, ureA, and cagA genes using PCR and high-throughput pyrosequencing analysis. Results: Forty-five percent (44/97) of patients tested positive for ureA and 42.3% (41/97) tested positive by a rapid urease test. One hundred percent (35/35) of patients who tested positive by both methods were observed to have the cagA gene. Among these 35 patients, 23S rRNA SNPs were present in 34.3% (12/35). Conclusions: Gastric wash-based PCR and a pyrosequencing assay were used to rapidly detect and estimate the number of 23S rRNA SNPs in clinical isolates of H. pylori. Not only is this a less invasive technique, but it can also diagnose drug resistance. Copyright (C) 2011 S. Karger AG, Basel