Pyridoacridine alkaloids inducing neuronal differentiation in a neuroblastoma cell line, from marine sponge Biemna fortis
Pyridoacridine alkaloids inducing neuronal differentiation in a neuroblastoma cell line, from marine sponge Biemna fortis
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DOI：
10.1016/s0968-0896(03)00086-5
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发表时间：
2003-05-01
影响因子：
3.5
通讯作者：
Kobayashi, M
中科院分区：
文献类型：
--
作者：
Aoki, S;Wei, H;Kobayashi, M
从印度尼西亚海洋海绵 Biemna fortis 中分离出一种新的和三种已知的吡啶吖啶生物碱，作为针对小鼠神经母细胞瘤细胞系 Neuro 2A 的神经元分化诱导剂。通过光谱研究和化学转化确定了新化合物 Labuanine A (1) 的化学结构。这些吡啶吖啶生物碱在 0.03-3 μM 浓度下诱导超过 50% 的细胞发生多极神经突发生。化合物 3 在其中表现出最强的神经突活性，还诱导乙酰胆碱酯酶（Neuro 2A 中的神经元标记物）增加，并将细胞周期阻滞在 G2/M 期。 (C) 2003 Elsevier Science Ltd. 保留所有权利。
A new and three known pyridoacridine alkaloids were isolated from the Indonesian marine sponge Biemna fortis as neuronal differentiation inducers against a murine neuroblastoma cell line, Neuro 2A. The chemical structure of the new compound, labuanine A (1), was determined by spectroscopic study and chemical conversion. These pyridoacridine alkaloids induced multipolar neuritogenesis in more than 50% of cells at 0.03-3 muM concentration. Compound 3, which showed the strongest neuritogenic activity among them, also induced increase of acetylcholinesterase, a neuronal marker in Neuro 2A and arrested cell cycle at the G2/M phase. (C) 2003 Elsevier Science Ltd. All rights reserved.