Biological activity of Barbados cherry (Acerola fruits, fruit of Malpighia emarginata DC) extracts and fractions
Biological activity of Barbados cherry (Acerola fruits, fruit of Malpighia emarginata DC) extracts and fractions
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DOI：
10.1002/ptr.1426
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发表时间：
2004-03-01
影响因子：
7.2
通讯作者：
Molnár, J
中科院分区：
文献类型：
--
作者：
Motohashi, N;Wakabayashi, H;Molnár, J
巴巴多斯樱桃的分级(针叶果，马利果属植物的果实)采用有机溶剂萃取法和柱层析法，采用两种提取方法进行提取。A4和A6组分(丙酮提取物)和H3和HE3组分(己烷提取物)具有较高的细胞毒活性。与正常细胞如人牙周膜成纤维细胞(HPLF)和人牙龈成纤维细胞(HGF)相比，这四个组分对人口腔鳞状细胞癌(HSC-2)和人颌下腺癌(HSG)等肿瘤细胞具有更强的细胞毒活性。HE2(正己烷提取物)、AE2(乙酸乙酯提取物)、AE3、AE4、AE5、A8、A9和A10对革兰氏阳性表皮葡萄球菌ATCC1228有较高的抗菌活性，而对革兰氏阴性杆菌代表菌株E.Coli和P.铜绿假单胞菌。这些组分对幽门螺杆菌、两种代表性的念珠菌和人类免疫缺陷病毒(HIV)没有活性。H3、H4和HE3具有较高的肿瘤特异性细胞毒作用，其多药耐药(MDR)逆转活性也高于阳性对照(+/-)-维拉帕米。ESR波谱显示，自由基介导的氧化不参与肿瘤特异性细胞毒活性的诱导。巴巴多斯樱桃的肿瘤特异性细胞毒活性和多药耐药逆转活性可能提示其在癌症治疗中的潜在应用。版权所有(C)2004 John Wiley Sons，Ltd.
Fractionation of barbados cherry (acerola fruit, a fruit of Malpighia emarginata DC.) extracts were performed by organic solvent extractions and column chromatographies, using two extraction methods. Higher cytotoxic activity was concentrated in fractions A4 and A6 (acetone extract), and H3 and HE3 (hexane extract). These four fractions showed higher cytotoxic activity against tumor cell lines such as human oral squamous cell carcinoma (HSC-2) and human submandibular gland carcinoma (HSG), when compared with that against normal cells such as human periodontal ligament fibroblasts (HPLF) and human gingival fibroblasts (HGF). HE2 (hexane extract), AE2 (ethyl acetate extract), AE3, AE4, AE5, A8, A9 and A10 showed some relatively higher anti-bacterial activity on the Gram-positive Staphylococcus epidermidis ATCC 1228 but were ineffective on the representative Gram-negative species E. coli and Ps. aeruginosa. The fractions were inactive against Helicobacter pylori, two representative Candida species, and human immunodeficiency virus (HIV). H3, H4 and HE3, which displayed higher tumor-specific cytotoxicity also showed higher multidrug resistance (MDR) reversal activity, than (+/-)-verapamil as positive control. ESR spectroscopy shows that the radical-mediated oxidation is not involved in the induction of tumor-specific cytotoxic activity. The tumor specific cytotoxic activity and MDR reversal activity of barbados cherry may suggest its possible application for cancer therapy. Copyright (C) 2004 John Wiley Sons, Ltd.