Quantum dot-related genotoxicity perturbation can be attenuated by PEG encapsulation
Quantum dot-related genotoxicity perturbation can be attenuated by PEG encapsulation
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PEG 封装可减弱量子点相关的遗传毒性扰动
DOI：
10.1016/j.mrgentox.2013.01.006
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发表时间：
2013-04-30
影响因子：
1.9
通讯作者：
Yang, Jun
中科院分区：
文献类型：
--
作者：
Ju, Li;Zhang, Guanglin;Yang, Jun
纳米材料与生物系统的相互作用正在成为阻碍纳米材料广泛采用的一个主要问题。使用不同尺寸的量子点（Qdot）（Qdot-440 nm 和 Qdot-680 nm）作为模型系统，我们研究了聚乙二醇（PEG）薄层表面修饰在减弱细胞系统中 Qdot 相关细胞毒性、遗传毒性扰动和氧化应激方面的作用。我们发现由核/壳 CdSe/ZnS 制成的未涂层 Qdots (U-Qdots) 确实可以诱导细胞毒性作用，包括抑制细胞生长。此外，中性彗星测定和 γ H2AX 焦点形成均表明 U-Qdots 以时间和剂量依赖性方式引起显着的 DNA 损伤。相比之下，细胞毒性分析和 γ H2AX 生成的结果表明，暴露于 PEG 包被的 Qdot 后对细胞的影响最小。没有观察到 PEG 涂层 Qdot 的毒性作用可能是由于 PEG 涂层可以抑制 U-Qdot 诱导的 ROS 产生。基于这些观察，我们得出结论，通过适当的表面修饰（例如 PEG 封装），Qdot 的遗传毒性可以显着降低。此外，PEG 封装也可作为减弱其他纳米颗粒纳米毒性的通用方法。 (C) 2013 Elsevier B.V. 保留所有权利。
Nanomaterial-biosystem interaction is emerging as a major concern hindering wide adoption of nanomaterials. Using quantum dots (Qdots) of different sizes (Qdot-440 nm and Qdot-680 nm) as a model system, we studied the effects of polyethylene glycol (PEG) thin-layer surface modification in attenuating Qdot-related cytotoxicity, genotoxicity perturbation and oxidative stress in a cellular system. We found that uncoated Qdots (U-Qdots) made of core/shell CdSe/ZnS could indeed induce cytotoxic effects, including the inhibition of cell growth. Also, both the neutral comet assay and gamma H2AX foci formation showed that U-Qdots caused significant DNA damage in a time- and dose-dependent manner. In contrast, results from cytotoxicity analysis and gamma H2AX generation indicate minimal impact on cells after exposure to PEG-coated Qdots. This lack of observed toxic effects from PEG-coated Qdots may be due to the fact that PEG-coating can inhibit ROS generation induced by U-Qdots. Based on these observations, we conclude that the genotoxicity of Qdots could be significantly decreased following proper surface modification, such as PEG encapsulation. In addition, PEG encapsulation may also serve as a general method to attenuate nanotoxicity for other nanoparticles. (C) 2013 Elsevier B.V. All rights reserved.