Comparative profiling of small RNAs of pig seminal plasma and ejaculated and epididymal sperm
Comparative profiling of small RNAs of pig seminal plasma and ejaculated and epididymal sperm
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猪精浆、射精和附睾精子小 RNA 的比较分析
DOI：
10.1530/rep-17-0014
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发表时间：
2017-06-01
期刊：
影响因子：
3.8
通讯作者：
Song, Chengyi
中科院分区：
文献类型：
--
作者：
Chen, Cai;Wu, Han;Song, Chengyi
精浆、附睾精子和射精精子中的小RNA的异同在很大程度上仍不清楚。我们对猪射精、附睾精和精浆中的小RNA图谱进行了系统的比较分析，发现小RNA物种的多样性分布基本相似，而小RNA的丰度在三个文库中差异很大；来自rRNA、tRNA、小核RNA、7SK RNA、NRON RNA和顺式调控RNA的miRNAs和小RNAs在三个文库中都很丰富，但没有piRNA。精液中大量的小RNA是精子特异的，只有8%-30%的小RNA与附睾精或精浆的小RNA重叠，一小部分(5%-18%)的小RNA在三个文库中共享，这表明精子RNA除了来自精巢外，还可能来自精浆、附睾液以及其他来源。大多数miRNAs是共同分布的，但在三个文库中有不同的表达，其中附睾精子的丰度最高，其次是射出的精子和精浆。对3个文库中前10位高表达miRNAs的靶基因预测表明，这些miRNAs可能参与精子发生、受精卵发育以及环境与动物的相互作用。我们的研究首次描述了精液、附睾精和精浆中小RNA图谱的异同，提示精子RNA可能起源于睾丸以外的器官。
The similarities and differences of small RNAs in seminal plasma, epididymal sperm and ejaculated sperm remain largely undefined. We conducted a systematic comparative analysis of small RNA profiles in pig ejaculated sperm, epididymal sperm and seminal plasma and found that the diversity distribution of small RNA species was generally similar, whereas the abundance of small RNAs is dramatically different across the three libraries; miRNAs and small RNAs derived from rRNA, tRNA, small nuclear RNA, 7SK RNA, NRON RNA and cis- regulatory RNA were enriched in the three libraries, but piRNA was absent. A large population of small RNAs from ejaculated sperm are ejaculated sperm specific, and only 8-30% of small RNAs overlapped with those of epididymal sperm or seminal plasma and a small proportion (5-18%) of small RNAs were shared in the three libraries, suggesting that, in addition to the testes, sperm RNAs may also originate from seminal plasma, epididymis as well as other resources. Most miRNAs were co- distributed but differentially expressed across the three libraries, with epididymal sperm exhibiting the highest abundance, followed by ejaculated sperm and seminal plasma. The prediction of target genes of the top 10 highly expressed miRNAs across the three libraries revealed that these miRNAs may be involved in spermatogenesis, zygote development and the interaction between the environment and animals. Our study provides the first description of the similarities and differences of small RNA profiles in ejaculated sperm, epididymal sperm and seminal plasma and indicates that sperm RNA may have origins other than the testes.