Leukocyte functional antigen 1 lowers T cell activation thresholds and signaling through cytohesin-1 and Jun-activating binding protein 1
Leukocyte functional antigen 1 lowers T cell activation thresholds and signaling through cytohesin-1 and Jun-activating binding protein 1
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DOI：
10.1038/ni984
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发表时间：
2003-11-01
影响因子：
30.5
通讯作者：
Nolan, GP
中科院分区：
文献类型：
--
作者：
Perez, OD;Mitchell, D;Nolan, GP
白细胞功能抗原-1(LFA-1)与细胞间黏附分子配体一起介导T细胞黏附，但其信号转导途径和功能作用尚不清楚。13维流式细胞仪细胞内磷酸蛋白染色显示，LFA-1激活诱导整合素链2的磷酸化和JUN激活结合蛋白1(JAB-1)的释放，并通过细胞粘蛋白-1介导激酶ERK1/2的信号转导。JAB-1和细胞粘附素-1的显性阴性抑制了IL-2的产生，并损害了T辅助细胞1型的分化。LFA-1刺激可降低T细胞活化阈值。因此，LFA-1信号有助于T细胞的激活和影响T细胞的分化。
Leukocyte functional antigen 1 (LFA-1), with intercellular adhesion molecule ligands, mediates T cell adhesion, but the signaling pathways and functional effects imparted by LFA-1 are unclear. Here, intracellular phosphoprotein staining with 13-dimensional flow cytometry showed that LFA-1 activation induced phosphorylation of the 2 integrin chain and release of Jun-activating binding protein 1 (JAB-1), and mediated signaling of kinase Erk1/2 through cytohesin-1. Dominant negatives of both JAB-1 and cytohesin-1 inhibited interleukin 2 production and impaired T helper type 1 differentiation. LFA-1 stimulation lowered the threshold of T cell activation. Thus, LFA-1 signaling contributes to T cell activation and effects T cell differentiation.