Regional Metabolic Patterns of Abnormal Postoperative Behavioral Performance in Aged Mice Assessed by 1H-NMR Dynamic Mapping Method
Regional Metabolic Patterns of Abnormal Postoperative Behavioral Performance in Aged Mice Assessed by 1H-NMR Dynamic Mapping Method
复制标题
1H-NMR动态绘图法评估老年小鼠术后行为表现异常的区域代谢模式
DOI：
10.1007/s12264-019-00414-4
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发表时间：
2020-01-01
影响因子：
5.6
通讯作者：
Guo, Xiangyang
中科院分区：
文献类型：
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作者：
Liu, Taotao;Li, Zhengqian;Guo, Xiangyang
术后神经行为功能异常（APNP）是术后早期的常见现象。麻醉和手术后脑内代谢物的稳态紊乱可能是APNP的重要原因。麻醉和手术后不同脑区代谢物的动态变化，以及它们与APNP的潜在联系仍不清楚。在这里，我们使用了一组行为测试来评估异氟烷麻醉下剖腹手术对老年小鼠的影响，并使用质子核磁共振（1H-NMR）光谱研究了不同时间点大脑12个不同子区域的代谢物。神经行为异常发生于麻醉/手术后6 h和/或9 h，24 h恢复正常。与对照组相比，模型组6 h时改变的代谢产物为天冬氨酸（Asp），差异主要表现在皮层; 9 h时显著改变主要表现在皮层和海马，对应的代谢产物为天冬氨酸和谷氨酸（Glu）。所有变化均在24 h时恢复至基线水平。急性APNP可能导致代谢的改变，皮层和海马中的代谢产物Asp和Glu可为理解APNP过程提供初步证据。
Abnormal postoperative neurobehavioral performance (APNP) is a common phenomenon in the early postoperative period. The disturbed homeostatic status of metabolites in the brain after anesthesia and surgery might make a significant contribution to APNP. The dynamic changes of metabolites in different brain regions after anesthesia and surgery, as well as their potential association with APNP are still not well understood. Here, we used a battery of behavioral tests to assess the effects of laparotomy under isoflurane anesthesia in aged mice, and investigated the metabolites in 12 different sub-regions of the brain at different time points using proton nuclear magnetic resonance (1H-NMR) spectroscopy. The abnormal neurobehavioral performance occurred at 6 h and/or 9 h, and recovered at 24 h after anesthesia/surgery. Compared with the control group, the altered metabolite of the model group at 6 h was aspartate (Asp), and the difference was mainly displayed in the cortex; while significant changes at 9 h occurred predominantly in the cortex and hippocampus, and the corresponding metabolites were Asp and glutamate (Glu). All changes returned to baseline at 24 h. The altered metabolic changes could have occurred as a result of the acute APNP, and the metabolites Asp and Glu in the cortex and hippocampus could provide preliminary evidence for understanding the APNP process.