Determination of gastric emptying in nonobese diabetic mice
Determination of gastric emptying in nonobese diabetic mice
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DOI：
10.1152/ajpgi.00317.2007
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发表时间：
2007-11-01
影响因子：
4.5
通讯作者：
Farrugia, Gianrico
中科院分区：
文献类型：
--
作者：
Choi, Kyoung Moo;Zhu, Jin;Farrugia, Gianrico
糖尿病胃轻瘫的动物研究由于不能跟踪同一小鼠胃排空的变化而受到限制。这项研究的目的是在非肥胖糖尿病(NOD)LTJ小鼠身上验证一种非致命性的胃排空方法，并研究随着年龄和早期糖尿病状态的顺序变化。对NOD LTJ小鼠进行了[C-13]辛酸呼气试验的可靠性和反应性测试，并用该试验测量了NOD LTJ小鼠和非肥胖糖尿病抵抗(NOR)LTJ小鼠的固体胃排空。13C呼气测试的结果类似于死后回收的一顿饭。苯乙酚促进胃排空[对照组：92+/-9min；苯乙酚：53+/-3min，平均半排空时间(T-1/2)+/-SE]，阿托品减慢胃排空(对照组：92+/-9min；阿托品：184+/-31min，平均T-1/2+/-SE)。非糖尿病NOD LTJ小鼠(8~12wk)正常胃排空(T-1/2)为91+/-2min。增龄对NOD LTJ和NOR LTJ小鼠的胃排空有不同的影响。糖尿病的发病伴随着糖尿病第1-2周胃排空的加速。胃排空在术后3~5周恢复正常，无延迟。[C-13]辛酸呼气试验准确测量NOD LTJ小鼠的胃排空，有助于研究糖尿病NOD LTJ小鼠胃排空变化的时间进程，并能够检测到糖尿病早期胃排空的加速。NOD LTJ和NOR LTJ小鼠之间胃排空的相反变化表明NOR LTJ小鼠不是研究NOD LTJ小鼠胃排空的良好对照。
Animal studies on diabetic gastroparesis are limited by inability to follow gastric emptying changes in the same mouse. The study aim was to validate a nonlethal gastric emptying method in nonobese diabetic ( NOD) LtJ mice, a model of type 1 diabetes, and study sequential changes with age and early diabetic status. The reliability and responsiveness of a [C-13] octanoic acid breath test in NOD LtJ mice was tested, and the test was used to measure solid gastric emptying in NOD LtJ mice and nonobese diabetes resistant (NOR) LtJ mice. The 13C breath test produced results similar to postmortem recovery of a meal. Bethanechol accelerated gastric emptying [ control: 92 +/- 9 min; bethanechol: 53 +/- 3 min, mean half emptying time (T-1/2) +/- SE], and atropine slowed gastric emptying ( control: 92 +/- 9 min; atropine: 184 +/- 31 min, mean T-1/2 +/- SE). Normal gastric emptying (T-1/2) in nondiabetic NOD LtJ mice ( 8 - 12 wk) was 91 +/- 2 min. Aging had differing effects on gastric emptying in NOD LtJ and NOR LtJ mice. Onset of diabetes was accompanied by accelerated gastric emptying during weeks 1 - 2 of diabetes. Gastric emptying returned to normal by weeks 3 - 5 with no delay. The [C-13] octanoic acid breath test accurately measures gastric emptying in NOD LtJ mice, is useful to study the time course of changes in gastric emptying in diabetic NOD LtJ mice, and is able to detect acceleration in gastric emptying early in diabetes. Opposing changes in gastric emptying between NOD LtJ and NOR LtJ mice suggest that NOR LtJ mice are not good controls for the study of gastric emptying in NOD LtJ mice.