Histological assessment of microtia cartilage, a potential source of autograft tissue in ear reconstruction.
Histological assessment of microtia cartilage, a potential source of autograft tissue in ear reconstruction.
复制标题
小耳软骨的组织学评估，耳重建中自体移植组织的潜在来源。
DOI：
10.1111/joa.14044
复制
发表时间：
2024
影响因子：
2.4
通讯作者：
Mariani,FrancescaV
中科院分区：
文献类型：
--
作者：
Lam,DavidD;Hegde,NeelV;Patel,DivyaD;Lakeland,DanielL;Guardino,Nicholas;Kochhar,Amit;Mariani,FrancescaV
软骨是一种强大而灵活的结缔组织，在我们的身体中具有多种形式和功能。虽然软骨表现出某些形式的有限修复，但在大多数情况下，它不是特别再生。因此，在患者需要软骨重建的情况下，外科医生可以使用自体移植物来替换缺失或受损的组织。来自身体不同区域的软骨组织表现出组织学差异，并且供应有限。因此，重要的是表征这些差异，以确定最合适的自体移植物来源。在小耳畸形的情况下，耳廓发育不全的先天性畸形，重建通常利用来自患者自身肋软骨的软骨。这存在潜在的发病风险。在这项研究中，我们评估了小耳软骨的组织学特征与正常的耳廓和肋软骨从人类患者接受手术切除。组织化学用于评价细胞结构、脂质含量和ECM含量。使用贝叶斯统计方法，我们确定，虽然肋软骨是标准的组织供体，小耳软骨的显微解剖更密切地反映了正常的耳软骨比肋软骨。因此，小耳软骨可以作为一个额外的水库软骨重建。
Cartilage is a strong and flexible connective tissue that has many forms and functions in our body. While cartilage exhibits some forms of limited repair, for the most part, it is not particularly regenerative. Thus, in situations where patients require cartilage reconstruction, surgeons may use autografts to replace missing or damaged tissue. Cartilage tissues from different regions of the body exhibit histological differences and are in limited supply. Thus, it is important to characterize these differences to determine the most appropriate autograft source. In the case of microtia, a congenital deformity where the pinna is underdeveloped, reconstruction commonly utilizes cartilage sourced from a patient's own costal cartilage. This presents a potential morbidity risk. In this study, we evaluate the histological characteristics of microtia cartilage compared with normal auricular and costal cartilage obtained from human patients undergoing surgical resection. Histochemistry was used to evaluate cellularity, lipid content, and ECM content. Using a Bayesian statistical approach, we determined that while costal cartilage is the standard tissue donor, the microanatomy of microtia cartilage more closely reflects normal auricular cartilage than costal cartilage. Therefore, microtia cartilage may serve as an additional reservoir for cartilage during reconstruction.