4TH-DERIVATIVE SPECTROPHOTOMETRY ANALYSIS OF TRYPTOPHAN ENVIRONMENT IN PROTEINS - APPLICATION TO MELITTIN, CYTOCHROME-C AND BACTERIORHODOPSIN
4TH-DERIVATIVE SPECTROPHOTOMETRY ANALYSIS OF TRYPTOPHAN ENVIRONMENT IN PROTEINS - APPLICATION TO MELITTIN, CYTOCHROME-C AND BACTERIORHODOPSIN
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DOI：
10.1111/j.1432-1033.1983.tb07540.x
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
PADROS, E
中科院分区：
文献类型：
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作者：
DUNACH, M;SABES, M;PADROS, E
本文应用四阶导数光谱法分析了N-乙酰-L-色氨酸酰胺及其与N-乙酰-L-酪氨酸乙酯混合物的溶剂效应。这些化合物在不同的介质中进行了分析。结果发现，四阶导数光谱的最长波长最小值的位置主要由Trp环境的性质决定，Tyr的贡献较小。还定义了一个几何参数，它取决于Trp和Tyr环境。同时考虑这两个参数允许的估计是由色氨酸环境。该方法适用于3个良好的特点蛋白质的构象变化的研究，蜂毒，细胞色素c从马心和细菌视紫红质从盐生盐杆菌。所获得的结果被发现是根据这些蛋白质的已知构象特性。此外，四阶导数操作完全消除了辅基近紫外波段的干扰。
Fourth-derivative spectrophotometry is applied to the analysis of solvent effects on the spectral transitions of N-acetyl-L-tryptophan amide, as well as of its mixtures with N-acetyl-L-tyrosine ethyl ester. These compounds were analyzed in different media. It was found that the position of the longest-wavelength minimum of the 4th-derivative spectrum is mainly determined by the nature of the Trp environment, with a minor contribution from that of Tyr. A geometrical parameter is also defined, which depends on both Trp and Tyr environments. The simultaneous consideration of both parameters allows an estimation to be made of the Trp environment. The method is applied to the study of conformational changes of 3 well-characterized proteins; melittin from bee venom, cytochrome c from horse heart and bacteriorhodopsin from Halobacterium halobium. The results obtained are found to be in accordance with the known conformational properties of these proteins. In addition, the 4th-derivative operation completely eliminates the interference from the near-UV bands of the prosthetic groups.