Brain GABA editing by localized in vivo 1H magnetic resonance spectroscopy
Brain GABA editing by localized in vivo 1H magnetic resonance spectroscopy
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DOI：
10.1002/nbm.863
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发表时间：
2004-04-01
影响因子：
2.9
通讯作者：
Durif, F
中科院分区：
文献类型：
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作者：
Bielicki, G;Chassain, C;Durif, F
H-1 MRS在特定脑区编辑GABA是一种独特的工具，用于体内非侵入性研究神经传递障碍。利用基于双量子相干（DQC）的序列实现了选择性GABA检测。我们的脉冲序列进行精确的测量，没有由于空间定位造成的伪影。该序列在一个幻影溶液上进行了测试。利用该方法测定了GABA转氨酶特异性抑制剂vigabatrin在大鼠脑中的作用，并在猴脑中进行了GABA的体内检测。大鼠被分成两组。在对照组，大鼠可以喝水，在另一组（vigabatrin， VGB，大鼠），动物可以自由地饮用含有vigabatrin的水。治疗3周后，麻醉大鼠进行体内核磁共振波谱研究。在实验结束时，大脑被迅速取出，冷冻夹住，用4%的高氯酸提取。用离子交换色谱-茚三酮检测法测定一部分酸提取物的GABA浓度。第二个用于高分辨率核磁共振分析。经色谱测定，对照组GABA浓度为1.23 +/- 0.06 μ mol/g， vigabatrin处理大鼠GABA浓度为4.89 +/- 1.60 μ mol/g。体内NMR结果与离体NMR结果（r = 0.99, p < 0.01）和色谱结果（r = 0.98, p < 0.01）密切相关。离体结果与色谱结果的相关性也很高（r = 0.99, p < 0.001）。该脉冲序列对位于非人类灵长类动物大脑右侧基底神经节区域的376个多体素进行了GABA编辑。因此，可以提出这种体内GABA编辑方案，用于精确测量脑GABA浓度。版权所有：John Wiley Sons， Ltd. 2004
Editing of GABA by H-1 MRS in a specific brain area is a unique tool for in vivo non-invasive investigation of neurotransmission disorders. Selective GABA detection is achieved using sequences based on double quantum coherence (DQC). Our pulse sequence makes accurate measurements without artefacts due to spatial localization. The sequence was tested on a phantom solution. The effect of vigabatrin, a specific inhibitor of GABA transaminase, was measured in rat brain and GABA detection was performed in vivo in monkey brain using this procedure. Rats were spilt into two groups. In the control group, the rats had access to water and, in the other group (vigabatrin, VGB, rats), animals were allowed free access to drinking water containing vigabatrin. After 3 weeks of treatment, rats were anesthetized for in vivo NMR spectroscopy investigation. At the end of the experiment, brains were quickly removed, freeze-clamped and extracted with 4% perchloric acid. One part of the acid extract was used for GABA concentrations assessment by ion exchange chromatography with ninhydrin detection. The second was used for high-resolution NMR analysis. By chromatography measurements, the GABA concentration was 1.23 +/- 0.06 mumol/g for controls, while for vigabatrin-treated rats the GABA concentration was 4.89 +/- 1.60 mumol/g. The NMR in vivo results were closely correlated with the NMR ex vivo (r = 0.99, p < 0.01) and chromatography results (r = 0.98, P < 0.01). The correlation between ex vivo results and chromatography results was also high (r = 0.99, p < 0.001). This pulse sequence performed GABA editing from a 376 mul voxel located on the right basal ganglia area in a non-human primate brain. This in vivo GABA editing scheme can thus be proposed for accurate measurement of brain GABA concentrations. Copyright (C) 2004 John Wiley Sons, Ltd.