EXPRESSION OF A HYBRID IMMUNOGLOBULIN-T CELL-RECEPTOR PROTEIN IN TRANSGENIC MICE
EXPRESSION OF A HYBRID IMMUNOGLOBULIN-T CELL-RECEPTOR PROTEIN IN TRANSGENIC MICE
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DOI：
10.1016/0092-8674(89)90943-4
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发表时间：
1989-09-08
期刊：
影响因子：
64.5
通讯作者：
HEDRICK, SM
中科院分区：
文献类型：
--
作者：
BECKER, MLB;NEAR, R;HEDRICK, SM
我们使用地高辛特异性抗体的 VDJH 外显子构建了混合免疫球蛋白 (VDJH)-T 细胞受体 (C.alpha.) 基因。该基因被用来培育一系列转基因小鼠。杂交VDJH-C.alpha。蛋白质在这些小鼠的 T 细胞子集上表达，我们已经证明，根据 CD3 和 TCR.beta 的共沉淀和共调节标准，它形成功能性 TCR 复合物的一部分。链组分和用抗独特型抗体或地高辛激活 T 细胞。此外，在表达杂合蛋白的细胞中，存在内源TCRα的等位基因排斥。基因。我们讨论了 T 细胞受体和免疫球蛋白的比较结构的含义。
We have constructed a hybrid immunoglobulin (VDJH)-T cell receptor (C.alpha.) gene using the VDJH exon from a digoxin-specific antibody. This gene was used to make a line of transgenic mice. The hybrid VDJH-C.alpha. protein is expressed on a subset of T cells in these mice, and we have shown that it forms part of a functional TCR complex by the criteria of coprecipitation and comodulation of CD3 and TCR.beta. chain components and T cell activation with anti-idiotypic antibodies or digoxin. Furthermore, in cells expressing the hybrid protein, there is allelic exclusion of endogenous TCR .alpha. genes. We discuss the implications for the comparative structure of T cell receptors and immunoglobulins.