THE RECOMBINATION ACTIVATING GENE-1 (RAG-1) TRANSCRIPT IS PRESENT IN THE MURINE CENTRAL-NERVOUS-SYSTEM
THE RECOMBINATION ACTIVATING GENE-1 (RAG-1) TRANSCRIPT IS PRESENT IN THE MURINE CENTRAL-NERVOUS-SYSTEM
复制标题
DOI：
10.1016/0092-8674(91)90220-s
复制
发表时间：
1991-01-11
期刊：
影响因子：
64.5
通讯作者：
BALTIMORE, D
中科院分区：
文献类型：
--
作者：
CHUN, JJM;SCHATZ, DG;BALTIMORE, D
重组激活基因rag-1和rag-2可能编码V(D)J位点特异性重组机制的组成部分。我们报道了用聚合酶链式反应、原位杂交和Northern印迹分析在小鼠中枢神经系统中检测到低水平的RAG-1转录本。相比之下，真实的RAG-2转录本不能在中枢神经系统中重复检测到。RAG-1转录本广泛存在于胚胎和出生后的神经元中，在出生后大脑中神经细胞密度高的区域(小脑和海马结构)转录最为明显。结果表明，RAG-1在神经元中发挥作用，其作用可能是特定地重组神经元基因组位置的元件，或者防止这些长期存活的细胞中基因组的有害变化。
The recombination activating genes, RAG-1 and RAG-2, are likely to encode components of the V(D)J site-specific recombination machinery. We report here the detection of low levels of the RAG-1 transcript in the murine central nervous system by polymerase chain reaction, in situ hybridization, and Northern blot analyses. In contrast, an authentic RAG-2 transcript could not be detected reproducibly in the central nervous system. The RAG-1 transcript was found to be widespread in embryonic and postnatal neurons, with transcription being most apparent in regions of the postnatal brain with a high neuronal cell density (the cerebellum and the hippocampal formation). The results suggest that RAG-1 functions in neurons, where its role might be to recombine elements of the neuronal genome site-specifically, or to prevent detrimental alterations of the genome in these long-lived cells.