Human plasma converting enzyme.
Human plasma converting enzyme.
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人血浆转化酶。
DOI：
10.1016/0003-9861(71)90518-2
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发表时间：
1971
影响因子：
3.9
通讯作者：
I. Wilson
中科院分区：
文献类型：
--
作者：
H. Lee;J. N. Larue;I. Wilson
用硫酸铵和凝胶硝化法从人血浆中纯化了部分血管紧张素转换酶。讨论了其与猪血浆转化酶的一些动力学和生理特性。人血浆转化酶的km值为4.5 × 10−5m。通过蔗糖密度梯度沉降技术获得了该酶的20，w值（8.2），估计人和猪血浆中转化酶的分子量相同，约为150,000道尔顿。该酶在人血浆中的最大活性约为4.5单位/ ml，计算出血管紧张素I转化为血管紧张素II的半衰期约为7分钟。在猪的血浆中，这种缓慢的速率表明，血管紧张素的“激活”一定比显而易见的更多。
Angiotensin-converting enzyme was partially purified from human plasma by ammonium sulfate and Sephadex gel nitration. Some of its kinetic and physiological properties were discussed in comparison with those of hog plasma-converting enzyme. TheKmvalue for human plasma converting enzyme was obtained as 4.5 × 10−5m. TheS20,Wvalue (8.2) for the enzyme was obtained by the sucrose density gradient sedimentation technique and the same molecular weight for the converting enzyme in human and hog plasma was estimated to be about 150,000 daltons. The maximum activity of the enzyme in human plasma is about 4.5 units per ml, and the calculated half-time for the conversion of angiotensin I to angiotensin II is about 7 min. This slow rate, as in hog plasma, suggests that there must be more to the “activation” of angiotensin than is immediately obvious.