In situ control of cellular growth and migration on substrates using microelectrodes
In situ control of cellular growth and migration on substrates using microelectrodes
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DOI：
10.1021/ja045702t
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发表时间：
2004-11-24
影响因子：
15
通讯作者：
Nishizawa, M
中科院分区：
文献类型：
--
作者：
Kaji, H;Tsukidate, K;Nishizawa, M
我们描述了一种电化学方法，直接生长和迁移的哺乳动物细胞的基板上在原位cultureinsituation.Exposing的白蛋白涂层基板的氧化剂，次溴酸，电化学产生的尖端的扫描microelectrode，局部切换基板从cytophobic细胞粘附。这种转化产生了细胞微图案的形成。由于表面处理所需的氧化剂的浓度不会对细胞培养物造成显著损害，因此我们能够通过在预先存在的细胞图案上绘制粘合剂微图案来指导原位细胞增殖和迁移。
We describe an electrochemical method to direct the growth and migration of mammalian cells on a substrate during cultivation in situ.Exposing the albumin-coated substrate to an oxidizing agent, hypobromous acid, electrochemically generated at the tip of the scanning microelectrode, locally switched the substrate from cytophobic to cell-adhesive. This transformation generated the formation of cellular micropatterns. Since the concentration of the oxidizing agent required for the surface processing did not cause significant damage to the cell cultures, we were able to direct in situ cellular proliferation and migration by drawing adhesive micropatterns over the preexisting cellular pattern.