Identification and pathogenicity of bacteria isolated from pome fruit trees in the Eastern Anatolia region of Turkey Identifizierung und Pathogenität von Bakterien aus ostanatolischen Kernobstbäumen
Identification and pathogenicity of bacteria isolated from pome fruit trees in the Eastern Anatolia region of Turkey Identifizierung und Pathogenität von Bakterien aus ostanatolischen Kernobstbäumen
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发表时间：
2006
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通讯作者：
Recep Kotan;F. Şahin;A. Ala
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作者：
Recep Kotan;F. Şahin;A. Ala
摘要1999年、2000年和2001年春季，从土耳其安纳托利亚东部地区五个不同省份种植的梨果中分离出细菌，包括可能的致病性和非致病性菌种。采用Sherlock微生物鉴定系统（MIS）对324株细菌进行鉴定，分属于36属76种。细菌种类最丰富的是欧文氏菌（16.9%），其次是假单胞菌pv。产碱杆菌（5.8%）、脱羧勒克莱尔氏菌（4%）和其他69种（26.5%）。来自12个不同物种的82个菌株是潜在的病原体，因为它们是过敏反应（HR）阳性的，并感染金冠苹果叶片。这是第一个研究表明，A。piechaudii，B. pumilus、紫色色杆菌（Chromobacterium violaceum）、E.中间体、大黄欧文氏菌、成团泛菌、铜绿假单胞菌、胡蒂假单胞菌、菊苣假单胞菌和恶臭假单胞菌是梨果的可能叶病原体，但需要进一步深入测试来确认这些初步结果。通过脂肪酸甲酯谱、代谢指纹、血清学和生化试验对可能的致病菌株进行了鉴定。然而，一些物种的鉴定结果之间存在差异。因此，我们的数据表明，一个单一的方法不能足够的植物病原细菌的鉴定。
Summary Bacteria including possible pathogenic and non-pathogenic species were isolated from pome fruits grown in five different provinces of the Eastern Anatolia region of Turkey during the springs 1999, 2000 and 2001. A total of 324 bacterial strains belonging to 76 species in 36 genera were identified by using Sherlock Microbial Identification System (MIS). The most abundant bacterial species was Erwinia amylovora (16.9%), followed by Pseudomonas syringae pv. syringae (16.6%), P. agglomerans (11.7%), Bacillus pumilus (10.8%), Enterobacter intermedius (7.7%), Alcaligenes piechaudii (5.8%), Leclercia adecarboxylata (4%) and 69 other species (26.5%). Eighty-two of the strains from 12 different species are potential pathogens because they were hypersensitive response (HR) positive and infected Golden Delicious apple leaves. This is the first study to show that A. piechaudii, B. pumilus, Chromobacterium violaceum, E. intermedius, Erwinia rhapontici, Pantoea agglomerans, Pseudomonas aeruginosa, Pseudomonas huttiensis, Pseudomonas cichorii and Pseudomonas putida are possible foliar pathogens of pome fruits but, further in-depth testing will be needed to confirm these preliminary results. Possible pathogenic strains were characterized by fatty acid methyl ester profiles, metabolic fingerprints, serological and biochemical tests. However, discrepancies were found among identification results for some species. Our data therefore suggest that a single approach can not be adequate for the identification of plant pathogenic bacteria.