Malaria sporozoites evade host complement attack
Malaria sporozoites evade host complement attack
复制标题
DOI：
10.1111/pim.13012
复制
发表时间：
2023-10-19
影响因子：
2.2
通讯作者：
Xu，Wenyue
中科院分区：
文献类型：
--
作者：
Jiao，Shiming;Tan，Nie;Xu，Wenyue
补体是宿主抵抗细菌和病毒感染的先天免疫反应的第一线；然而，它在疟疾肝脏阶段的发展中的作用尚不清楚。我们发现，蚊虫叮咬或静脉注射感染子孢子激活了系统补体，但C3的耗尽和C3的敲除都不会对疟疾肝期的发育产生显著影响。小鼠血清与经胰酶处理的子孢子孵育，但不与幼子孵育，可在子孢子表面沉积膜攻击复合体(MAC)，并极大地减少红外型(EEF)的数量。进一步的研究表明，补体H因子(CFH)的募集可能与阻止MAC沉积在幼子孢子表面有关。我们的数据有力地表明，子孢子可以逃避补体攻击，并为我们提供了一种预防疟疾感染的新策略。
Complement is the first line of the host innate immune response against bacterial and viral infections; however, its role in the development of the malaria liver stage remains undefined. We found that sporozoite infection by either a mosquito bite or intravenous injection activated systemic complement, but neither depletion of C3 nor knockout of C3 had a significant effect on malaria liver stage development. Incubation of mouse serum with trypsin‐treated sporozoites, but not naive sporozoites, led to the deposition of a membrane attack complex (MAC) on the surface of sporozoites and greatly reduced the number of exo‐erythrocytic forms (EEF). Further studies have shown that the recruitment of complement H factor (CFH) may be associated with the prevention of MAC deposition on the surface of naïve sporozoites. Our data strongly suggest that sporozoites can escape complement attacks and provide us with a novel strategy to prevent malaria infection.