Exposure to ionizing radiation modifies neurally-evoked electrolyte transport and some inflammatory responses in rat colon in vitro
Exposure to ionizing radiation modifies neurally-evoked electrolyte transport and some inflammatory responses in rat colon in vitro
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DOI：
10.1080/095530098142743
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发表时间：
1998-01-01
影响因子：
2.6
通讯作者：
Griffiths, NM
中科院分区：
文献类型：
--
作者：
François, A;Aigueperse, J;Griffiths, NM
目的：描述大鼠急性暴露于伽马辐射后结肠对神经刺激的反应性，并将观察到的变化与一些参数联系起来。材料与方法：将大鼠分别置于5、10 Gy的Cs-137 γ射线下（假照射），分别于照射后1、3、7 d进行研究。将剥离的结肠段安装在ususe腔中，用于测量神经诱发的电解质运输（电场刺激）。结肠组织进行生化分析（组织5-羟色胺、组胺、白三烯B-4、一氧化氮合酶）和组织学分析（肥大细胞）。结果：受辐射大鼠结肠近端和远端在第1天和第3天对电场刺激反应低下，但在第7天恢复。在远端结肠中，10 Gy后1天，氨基酚反应减弱。肥大细胞、组织组胺和白三烯B的合成在所有时间点均显著降低，但5-HT和诱导NOS活性未见变化。结论：辐照后大鼠结肠对神经刺激反应降低。最初（1和3天）的反应与肥大细胞和组胺减少相关，但在第7天则不是。
Purpose: To characterize the responsiveness of the colon to neural stimulation following acute exposure of rats to gamma-radiation and to correlate observed changes to a number of parameters.Materials and methods: Rats were exposed to 5 or 10 Gy Cs-137 gamma- radiation or not (sham-irradiated) and studied at 1, 3 and 7 days after irradiation. Stripped segments of colon were mounted in Ussing chambers for measurements of neurally-evoked electrolyte transport (electrical field stimulation). Colonic tissue was also taken for biochemical (tissue 5-hydroxytryptamine, histamine, leukotriene B-4, nitric oxide synthase) and histological analyses (mast cells).Results: In irradiated rats both proximal and distal colon were hyporesponsive to electrical field stimulation at 1 and 3 days, but had recovered by 7 days. In the distal colon, carbachol responses were attenuated 1 day after 10 Gy. Mast cells, tissue histamine and leukotriene B, synthesis were significantly reduced at all time points but no changes were seen in 5-HT or inducible NOS activity.Conclusions: Rat colon becomes hyporesponsive to neural stimuli post-irradiation. The response initially (1 and 3 days) correlates with decreased mast cells and histamine, but not at 7 days.