Transwell In Vitro Cell Migration and Invasion Assays.
Transwell In Vitro Cell Migration and Invasion Assays.
复制标题
在体外细胞迁移和侵袭测定法。
DOI：
10.1007/978-1-0716-3052-5_22
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
细胞迁移和侵袭在正常生理和疾病中都起着重要的作用。因此，评估细胞迁移和侵袭能力的方法对于阐明正常细胞过程和疾病的潜在机制是必要的。在这里，我们描述了常用的transwell体外方法研究细胞迁移和侵袭。跨井迁移试验涉及在使用两个介质填充的室建立化学引诱剂梯度后，细胞通过多孔膜的趋化性。跨井侵袭试验包括在多孔膜上添加细胞外基质，多孔膜只允许具有侵袭特性的细胞（如肿瘤细胞）趋化。
Cell migration and invasion have essential roles in both normal physiology and disease. As such, methodologies to assess cell migratory and invasive capacities are necessary to elucidate normal cell processes and underlying mechanisms of disease. Here, we describe commonly used transwell in vitro methods for the study of cell migration and invasion. The transwell migration assay involves the chemotaxis of cells through a porous membrane after the establishment of a chemoattractant gradient using two medium-filled compartments. The transwell invasion assay involves the addition of an extracellular matrix on top of the porous membrane which only permits chemotaxis of cells which possess invasive properties such as tumor cells.