Reconstitution of chromatin: assembly of the nucleosome.
Reconstitution of chromatin: assembly of the nucleosome.
复制标题
染色质的重建：核小体的组装。
DOI：
10.1093/nar/5.2.505
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发表时间：
1978
影响因子：
14.9
通讯作者：
M. Daune
中科院分区：
文献类型：
--
作者：
F. Wilhelm;M. Wilhelm;M. Erard;M. Daune
确定了染色质重组过程中四种组蛋白H_2A、H_2B、H_3和H_4与DNA重新结合的顺序。在重组的每一步，DNA和相关的组蛋白通过甘油梯度离心法从游离组蛋白中分离出来。用凝胶电泳法分析未结合和重新结合的组蛋白，用圆二色谱和电子显微镜分析组蛋白-DNA复合体。我们发现H3和H4在1.2M和0.85M的氯化钠溶液中首先与DNA结合，并形成核小体结构；在第二步中，组蛋白H_2A和H_2B被放置在核周围以完成核小体。
The order of reassociation of the four histones H2a, H2b, H3 and H4 to the DNA during the reconstitution of chromatin was determined. At each step of the reconstitution the DNA and associated histones were separated from the free histones by centrifugation in a glycerol gradient. The unbound and reassociated histones were analysed by gel electrophoresis and the histone-DNA complexes characterized by circular dichroism and electron microscopy. We show that H3 and H4 bind first to the DNA between 1.2 M NaCl and 0.85 M NaCl and impose a nucleosome like structure; in a second step histones H2a and H2b are placed around this kernel to complete the nucleosome.