Organization and sequences of p-hydroxybenzaldehyde dehydrogenase and other plasmid-encoded genes for early enzymes of the p-cresol degradative pathway in Pseudomonas putida NCIMB 9866 and 9869
Organization and sequences of p-hydroxybenzaldehyde dehydrogenase and other plasmid-encoded genes for early enzymes of the p-cresol degradative pathway in Pseudomonas putida NCIMB 9866 and 9869
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DOI：
10.3109/10425179909033930
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发表时间：
1999-01-01
期刊：
影响因子：
--
通讯作者：
McIntire, WS
中科院分区：
文献类型：
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作者：
Cronin, CN;Kim, J;McIntire, WS
恶臭假单胞菌NCIMB 9866和9869降解对甲酚所产生的对羟基苯甲醛的代谢所需的乙醛脱氢酶基因(命名为pchA)已分别在pRA4000和pRA500上被鉴定。该基因位于pchC和pchF基因的上游，编码对甲酚甲基羟基酶(PCMH)的亚基，对甲酚降解途径中的前一种酶。在pRA500中，后一个基因后面是编码原儿茶酸-3，4-双加氧酶的α(PcaG)和β(PCAH)亚基的基因，而在pRA4000中，编码PCMH的基因后面是一个开放阅读框，编码的蛋白质与产碱假单胞菌成熟酶相关的蛋白相似。在两个质粒中的pchC和pchF基因之间发现了一个编码未知功能蛋白的基因，命名为pchX。
The gene (designated pchA) encoding the aldehyde dehydrogenase that is required to metabolise the p-hydroxybenzaldehyde produced by the degradation of p-cresol in Pseudomonas putida NCIMB 9866 and 9869 has been identified on plasmids pRA4000 and pRA500, respectively. The gene lies immediately upstream of the pchC and pchF genes encoding the subunits of p-cresol methylhydroxylase (PCMH), the preceeding enzyme in the p-cresol degradative pathway. In pRA500 the latter genes are followed by the genes encoding the alpha (pcaG) and beta (pcaH) subunits of protocatechuate-3,4-dioxygenase, whereas in pRA4000 the genes encoding PCMH are followed by an open reading frame encoding a protein that is similar to the maturase-related protein of P. alcaligenes. A gene, designated pchX, that encodes a protein of unknown function was identified between the pchC and pchF genes in both plasmids.