Bioavailability of genistein and its glycoside genistin as measured in the portal vein of freely moving unanesthetized rats
Bioavailability of genistein and its glycoside genistin as measured in the portal vein of freely moving unanesthetized rats
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DOI：
10.1021/jf060783t
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发表时间：
2006-10-18
影响因子：
6.1
通讯作者：
Rietjens, Ivonne M. C. M.
中科院分区：
文献类型：
--
作者：
Steensma, Aukje;Faassen-Peters, Maria A. W.;Rietjens, Ivonne M. C. M.
本研究描述了染料木素及其糖苷染料木素的体内生物利用度实验，无论是作为纯化合物还是从大豆分离蛋白提取物中，使用自由移动的未麻醉大鼠门静脉插管。结果表明，染料木素具有良好的生物利用度，给药后15分钟，首次检测时即可在门静脉血浆中观察到染料木素。染料木素、染料木素和浓缩蛋白大豆提取物的总染料木素及其结合物的AUC(0-24小时)分别为54、24和13Mh。这些结果表明染料木素对苷元的生物利用度高于其糖苷。染料木素以其糖苷形式被部分吸收。结论：基于门静脉血浆水平的生物利用度研究有助于深入了解肠和肝脏在糖基化类黄酮的脱糖和摄取特性中的作用。
The present study describes an in vivo bioavailability experiment for genistein and its glycoside genistin, either as pure compounds or from a soy protein isolate extract, using freely moving unanesthetized rats with a cannulation in the portal vein. The results show that genistein is readily bioavailable, being observed in portal vein plasma at the first point of detection at 15 min after dosing. The AUC(0-24h) values for total genistein and its conjugates were 54, 24, and 13 mu M h for genistein, genistin, and an enriched protein soy extract, respectively. These results indicate that the bioavailability of genistein is higher for the aglycon than for its glycoside. Genistin is partly absorbed in its glycosidic form. It is concluded that bioavailability studies based on portal vein plasma levels contribute to insight into the role of the intestine and liver in deglycosylation and uptake characteristics of glycosylated flavonoids.