Can polychlorinated biphenyl (PCB) signatures and enantiomer fractions be used for source identification and to age date occupational exposure?
Can polychlorinated biphenyl (PCB) signatures and enantiomer fractions be used for source identification and to age date occupational exposure?
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DOI：
10.1016/j.envint.2015.04.006
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发表时间：
2015-08
影响因子：
11.8
通讯作者：
D. Megson;J. Focant;D. Patterson;M. Robson;M. Lohan;P. Worsfold;S. Comber;R. Kalin;E. Reiner-E.-Reine
中科院分区：
文献类型：
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作者：
D. Megson;J. Focant;D. Patterson;M. Robson;M. Lohan;P. Worsfold;S. Comber;R. Kalin;E. Reiner-E.-Reine
详细的多氯联苯（PCB）的签名和CB-95，CB-136和CB-149的手性对映体分数（EF）的测量30名工人在Transformer拆卸厂。这是为了确定接触源，并调查不同接触期内多氯联苯特征和EFs的变化。提取约1.5 g血清，通过综合二维气相色谱-飞行时间质谱（GC × GC-TOFMS）分析创建PCB特征，并通过气相色谱-高分辨率质谱（GC-HRMS）分析计算EF。在血清样本中共发现了84种多氯联苯，其中7种指示性多氯联苯的浓度范围为11-350 ng g− 1血清（1.2-39 μg g g− 1脂质）。使用主成分分析（PCA），这是能够区分工人的背景或最近的最低限度的接触与长期职业接触的多氯联苯的签名进行了解释。在职业上接触多氯联苯的个人对多氯联苯Aroclor A1260的情况相似。然而，长期接触多氯联苯的人体内几种易受代谢影响的多氯联苯同源物（CB-95、CB-101和CB-151）的比例减少，而抵抗代谢的多氯联苯（CB-74、CB-153、CB-138和CB-180）的比例增加。研究结果还发现，第三组工人的CB-28、CB-60、CB-66、CB-74、CB-105和CB-118比例较高，他们似乎接触了额外的多氯联苯来源。结果显示，在一些样品中CB-95 E2对映体几乎完全清除，表明在人体中发生一种对映体的生物选择性代谢或优先排泄。通过沿着考虑多氯联苯浓度和详细的同类物特定特征，可以识别不同的暴露源，并深入了解暴露的幅度和持续时间。
Detailed polychlorinated biphenyl (PCB) signatures and chiral Enantiomer Fractions (EFs) of CB-95, CB-136 and CB-149 were measured for 30 workers at a transformer dismantling plant. This was undertaken to identify sources of exposure and investigate changes to the PCB signature and EFs over different exposure periods. Approximately 1.5 g of serum was extracted and PCB signatures were created through analysis by comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry (GC × GC–TOFMS) and EFs calculated following analysis by gas chromatography with high resolution mass spectrometry (GC–HRMS). A total of 84 PCBs were identified in the serum samples with concentrations of the 7 indicator PCBs ranging from 11–350 ng g− 1of serum (1.2–39 μg g− 1lipid). The PCB signatures were interpreted using principal component analysis (PCA) which was able to distinguish workers with background or recent minimal exposure from those with prolonged occupational exposure. Occupationally exposed individuals had a similar PCB profile to Aroclor A1260. However, individuals with prolonged exposure had depleted proportions of several PCB congeners that are susceptible to metabolism (CB-95, CB-101 and CB-151) and elevated proportions of PCBs that are resistant to metabolism (CB-74, CB-153, CB-138 and CB-180). The results also identified a third group of workers with elevated proportions of CB-28, CB-60, CB-66, CB-74, CB-105 and CB-118 who appeared to have been exposed to an additional source of PCBs. The results show near complete removal of the CB-95 E2 enantiomer in some samples, indicating that bioselective metabolism or preferential excretion of one enantiomer occurs in humans. By considering PCB concentrations along with detailed congener specific signatures it was possible to identify different exposure sources, and gain an insight into both the magnitude and duration of exposure.