Graphene Oxide and Lysozyme Ultrathin Films with Strong Antibacterial and Enhanced Osteogenesis
Graphene Oxide and Lysozyme Ultrathin Films with Strong Antibacterial and Enhanced Osteogenesis
复制标题
氧化石墨烯和溶菌酶超薄膜具有强抗菌和增强成骨作用
DOI：
10.1021/acs.langmuir.9b00035
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发表时间：
2019-05-21
期刊：
影响因子：
3.9
通讯作者：
Guan, Yifu
中科院分区：
文献类型：
--
作者：
Li, Meng;Li, Huaqiong;Guan, Yifu
全球对骨相关植入材料有巨大需求。当前植入材料存在慢性感染和骨愈合不良的缺陷，限制了其临床应用。在植入材料上用抗菌和成骨薄膜对植入表面进行功能化，为制造新型植入材料提供了新机遇。在本研究中，利用逐层（LBL）技术，通过氧化石墨烯（GO）和溶菌酶（Lys）交替沉积，制备了几十纳米厚的超薄（GO/Lys）₈薄膜。椭圆偏振光谱、紫外 - 可见光谱和傅里叶变换红外数据表明（GO/Lys）ₙ薄膜的沉积呈现连续增长，并且用原子力显微镜对该薄膜的物理性质（形态、粗糙度和刚度）进行了表征。超薄薄膜对革兰氏阳性金黄色葡萄球菌和革兰氏阴性大肠杆菌的杀菌效果显著，并提高了成骨分化效率，显示出在骨植入涂层中的潜在应用。我们相信这种LBL组装策略将为制造双功能表面铺平道路，并在未来指导植入表面的设计。
There is a great demand worldwide for bone-related implant materials. The drawbacks of chronic infections and poor bone healing of current implant materials have limited their clinical applications. Functionalizing the implant surfaces with antibacterial and osteogenic films on implant materials provides new opportunities for fabricating novel implant materials. In the present study, an ultrathin (GO/Lys)(8) film of several tens of nanometers was fabricated using a layer-by-layer (LBL) technique with alternative deposition of graphene oxide (GO) and lysozyme (Lys). The deposition of the (GO/Lys)(n) film exhibited a successive growth as supported by ellipsometry, UV-vis, and Fourier transform infrared data, and the physical properties (morphology, roughness, and stiffness) of this film were characterized with an atomic force microscope. The ultrathin films exhibited a great effect on bacterium sterilization of Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli and enhanced osteogenic differentiation efficiency, showing the potential application in bone implant coatings. We believe that this LBL assembling strategy will pave the way for fabricating dual-functional surfaces and guide the design of the implanted surfaces in the future.