Reply to the Letter: Increased bioavailability of plasma polyphenols via the intestinal fermentation of soybean fibers: a role for gut microbiome?
Reply to the Letter: Increased bioavailability of plasma polyphenols via the intestinal fermentation of soybean fibers: a role for gut microbiome?
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回复信件：通过大豆纤维肠道发酵提高血浆多酚的生物利用度：肠道微生物组的作用？
DOI：
10.1007/s00394-019-02030-4
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发表时间：
2019
期刊：
影响因子：
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通讯作者：
Hara H.
中科院分区：
文献类型：
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作者：
Trakooncharoenvit A;Tanaka S;Mizuta E;Hira T;Hara H.
尊敬的塞尔瓦托·奇伦博罗教授，首先，我们感谢您对我们最近的论文发表的评论和推荐的参考文献。我们也同意，槲皮素可能是肠道微生物释放这种分解代谢物的分子点火。根据您的建议和证据，栎素降解产物导致丙酸和其他有机酸的形成[1]。然而，我们想补充的解释是，与发酵膳食纤维产生的有机酸相比，槲皮素分解代谢物不是盲肠有机酸的大多数[2，3]。这一说法得到了我们盲肠有机酸结果的证实。Q组的有机酸浓度低于大豆纤维(QS)组，尤其是丙酸浓度[4]。这表明，盲肠有机酸主要是由盲肠发酵的膳食纤维造成的，而不是由槲皮素降解造成的。然而，我们计划进行另一项实验，用正常饮食，或含有大豆纤维的正常饮食，或同时含有大豆纤维和槲皮素的正常饮食喂养大鼠。本实验的结果可能会为我们提供更多的证据来解释这种纤维在提高槲皮素生物利用度方面的作用。您提到了肠道微生物区系在槲皮素代谢和血浆中黄酮醇生物利用度中的重要作用。我们推测，盲肠细菌可能上调了几个与碳水化合物代谢酶相关的基因的表达，其中膳食纤维在它们的生长过程中被用作底物[5-7]。因此，槲皮素及其代谢物避开了细菌的降解，并在血浆中升高。根据您对IGLEWICS和Hoaglin关于多个异常值的稳健检验的建议，我们根据来自IGlewicz和Hoaglin手册的公式对我们的数据进行了检验[8]。在我们的原始数据的计算中没有观察到异常值，所以我们可以确认我们的数据集可以使用。
Dear Prof Salvatore Chirumbolo, PhD First, we would like to thank you for your comments on our recent paper and the suggested references. We also agree that quercetin might act as a molecular ignition for gut microbiome to release such catabolites. From your suggestion and evidence, quercetin degraded products led to the formation of propionic and other organic acids [1]. However, we would like to add the explanation that quercetin catabolites are not the majority of the cecal organic acids when compared to organic acids produced from the fermentation of dietary fiber [2, 3]. This statement is confirmed by our cecal organic acids result. Organic acid concentrations of Q group are lower than that of soybean fiber-treated (QS) group, especially propionic concentration [4]. This suggests that cecal organic acids were mainly caused by cecal fermentation of dietary fibers and not quercetin degradation. However, we have planned to perform another experiment by feeding rats with normal diet, or normal diet containing soybean fiber, or normal diet containing both soybean fiber and quercetin. Results from this experiment might give us more evidence to explain the effect of this fiber on the enhancement in quercetin bioavailability. You have mentioned the important role of gut microflora in quercetin metabolism and flavonol bioavailability in plasma. We hypothesized that cecal bacteria might have upregulated the expression of several genes related to carbohydrate-metabolizing enzymes, wherein the dietary fibers were used as a substrate during their growth [5–7]. Consequently, quercetin and its metabolites had evaded bacterial degradation and had elevated within plasma. Upon your suggestion on Iglewics and Hoaglin’s robust test for multiple outliers, we have performed it on our data according to the formulation from Iglewicz and Hoaglin’s handbook [8]. No outliers were observed in the calculations on our raw data, so we can confirm that our data set could be used.