Evaluation of quantitative PCR and culture methods for detection of house dust fungi and streptomycetes in relation to moisture damage of the house
Evaluation of quantitative PCR and culture methods for detection of house dust fungi and streptomycetes in relation to moisture damage of the house
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DOI：
10.1111/j.1472-765x.2008.02431.x
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发表时间：
2008-10-01
影响因子：
2.4
通讯作者：
Nevalainen, A.
中科院分区：
文献类型：
--
作者：
Lignell, U.;Meklin, T.;Nevalainen, A.
目的：采用培养和定量聚合酶链反应（qPCR）的方法，分析了71份吸尘样品中的微生物浓度，并研究了微生物浓度与房屋受潮程度的关系。方法与结果：qPCR测得的微生物浓度与培养法测得的微生物浓度相关，但其数量级更高。qPCR的敏感性也较好。用qPCR测定的室内灰尘中几种微生物的浓度与房屋中水分损害的程度有关。这种关联在短孔青霉菌（Penicillium breviccompacactum）中最强，这是qPCR检测到的浓度最高的真菌之一。此外，sewalleia sebi、Trichoderma viride、Cladosporium sphaerspermum、Eurotium amstelodami以及青霉菌、曲霉菌和各种拟青霉联合试验组的室内尘埃浓度与水分损害程度显著相关。结论：这些菌种或测定组可作为室内湿害的指标。本研究的意义和影响：这一发现表明了qPCR方法的优势，该方法足够敏感，可以揭示受潮房屋与未受潮房屋间室内灰尘微生物浓度的差异。
Aims: Microbial concentrations in vacuumed house dust samples (n = 71) were analysed by culture and quantitative polymerase chain reaction (qPCR) methods and their association with extent of moisture damage in the house was studied.Methods and Results: Microbial concentrations measured by qPCR correlated with concentrations obtained by culture method, but were orders of magnitude higher. qPCR also had better sensitivity. Concentrations of several microbes in house dust, determined with qPCR, were associated with the extent of moisture damage in the house. This association was strongest for Penicillium brevicompactum, one of the fungi detected in highest concentrations by qPCR. Furthermore, house dust concentrations of Wallemia sebi, Trichoderma viride, Cladosporium sphaerospermum, Eurotium amstelodami and the combined assay group for Penicillium spp., Aspergillus spp. and Paecilomyces variotii were significantly associated with the extent of the moisture damage.Conclusion: These species or assay groups could probably be used as indicators of moisture damage in the house.Significance and Impact of the Study: This finding indicates the benefits of the qPCR method, which is sensitive enough to reveal the differences in microbial concentrations of house dust between moisture-damaged and undamaged houses.