Host-endoparasitoid-endosymbiont relationships: concealed Strepsiptera provide new twist to Wolbachia in Australian tephritid fruit flies.
Host-endoparasitoid-endosymbiont relationships: concealed Strepsiptera provide new twist to Wolbachia in Australian tephritid fruit flies.
复制标题
宿主-内寄生物-内共生关系：隐藏的链翅目为澳大利亚实蝇中的沃尔巴克氏体提供了新的转折。
DOI：
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发表时间：
2021
影响因子：
5.1
通讯作者：
M. Riegler
中科院分区：
文献类型：
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作者：
Sharon Towett;J. Morrow;Shannon Close;J. Royer;M. Riegler
沃尔巴克氏体是广泛存在的内共生生物，影响节肢动物的繁殖和适应性。沃尔巴克氏体主要是母系遗传，偶尔也会水平转移。此前，在澳大利亚的7种伤寒中报道了两种沃尔巴克氏体菌株的低流行率和滴度，可能表明频繁的水平转移。在这里，我们对野外捕获的沃尔巴克氏体阳性苍蝇进行了全基因组测序。出乎意料的是，我们发现了一种内寄生的链虫，双翅虫的完整有丝分裂基因组，这表明沃尔巴克氏体在果蝇中与隐性寄生有关。我们进行了第一次遗传表征，并在沃尔巴克氏体阳性的苍蝇中检测到达西弓形虫，但大多数沃尔巴克氏体阴性的苍蝇都是达西弓形虫阴性，这可能反映了达西弓形虫感染沃尔巴克氏体的多态性。我们从柱头苍蝇中解剖了达西布氏菌，证实了沃尔巴克氏菌感染达西布氏菌，但也在柱头苍蝇组织中发现了沃尔巴克氏菌，可能是体细胞的、水平转移的、非遗传的感染。此外，没有检测到沃尔巴克氏体cif和wmk基因，并且在整个分布的达西疟原虫中有丝分裂基因组变异非常低。因此，沃尔巴克氏体可能在没有生殖操作的情况下影响宿主的适应性。我们对13种绦虫物种的研究强调，隐藏的早期阶段的链虫寄生导致了之前沃尔巴克氏体共同感染的错误分配，模糊了这种常见的内共生生物的生态学研究及其通过寄生蜂的水平传播。这篇文章受版权保护。版权所有。
Wolbachia are widespread endosymbionts that affect arthropod reproduction and fitness. Mostly maternally inherited, Wolbachia are occasionally transferred horizontally. Previously, two Wolbachia strains were reported at low prevalence and titres across seven Australian tephritid species, possibly indicative of frequent horizontal transfer. Here, we performed whole-genome sequencing of field-caught Wolbachia-positive flies. Unexpectedly, we found complete mitogenomes of an endoparasitic strepsipteran, Dipterophagus daci, suggesting that Wolbachia in the flies are linked to concealed parasitisation. We performed the first genetic characterisation and detected D. daci in Wolbachia-positive flies not visibly parasitised, but most Wolbachia-negative flies were D. daci-negative, presumably reflecting polymorphism for the Wolbachia infections in D. daci. We dissected D. daci from stylopised flies and confirmed that Wolbachia infects D. daci, but also found Wolbachia in stylopised fly tissues, likely somatic, horizontally transferred, non-heritable infections. Furthermore, no Wolbachia cif and wmk genes were detected, and very low mitogenomic variation in D. daci across its distribution. Therefore, Wolbachia may influence host fitness without reproductive manipulation. Our study of 13 tephritid species highlights that concealed early stages of strepsipteran parasitisation led to the previous incorrect assignment of Wolbachia co-infections to tephritid species, obscuring ecological studies of this common endosymbiont and its horizontal transmission by parasitoids. This article is protected by copyright. All rights reserved.