Capsular serotype K1 or K2, rather than magA and rmpA, is a major virulence determinant for Klebsiella pneumoniae liver abscess in Singapore and Taiwan
Capsular serotype K1 or K2, rather than magA and rmpA, is a major virulence determinant for Klebsiella pneumoniae liver abscess in Singapore and Taiwan
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DOI：
10.1128/jcm.01150-06
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发表时间：
2007-02-01
影响因子：
9.4
通讯作者：
Koh, Tse-Hsien
中科院分区：
文献类型：
--
作者：
Yeh, Kuo-Ming;Kurup, A.;Koh, Tse-Hsien
荚膜血清型、magA 和 rmpA 已被记录为肺炎克雷伯菌肝脓肿的高患病率。为了研究荚膜血清型、magA 和 rmpA 与毒力的区域差异和相关性，在新加坡和台湾收集了 73 株分离株。通过逆流免疫电泳测定荚膜血清型，通过PCR测定magA和rmpA的存在，并通过吞噬作用和小鼠接种测定毒力。来自新加坡的分离株在基因组异质性、血清型流行率以及 magA 和 rmpA 的存在方面与来自台湾的分离株相似。最常见的血清型是 K1（34/73；46.6%），其次是 K2（15/73；20.5%）。 magA 仅限于 K1 血清型。所有 K1 或 K2 分离株以及 66.7% (16/24) 既不是 K1 血清型也不是 K2 血清型（非 K1/K2）的分离株携带 rmpA。血清型 K1 或 K2 分离株表现出比非 K1/K2 分离株的 rmpA 阳性和阴性组明显更高的吞噬细胞抗性和毒力。在非K1/K2组中，通过吞噬试验和小鼠模型，来自台湾和新加坡的rmpA阳性菌株的毒力特征不同，表明rmpA以外的因素对毒力有影响。新加坡和台湾的肺炎克雷伯菌肝脓肿的特征相似。荚膜血清型 K1 或 K2 在确定肺炎克雷伯菌肝脓肿的毒力方面比 magA 和 rmpA 发挥更重要的作用。
Capsular serotypes, magA, and rmpA have been documented in high prevalence for Klebsiella pneumoniae liver abscess. To investigate the regional difference and the correlation of capsular serotype, magA, and rmpA with virulence, 73 isolates were collected in Singapore and Taiwan. Capsular serotypes were determined by countercurrent immunoelectrophoresis, the presence of magA and rmpA was determined by PCR, and virulence was determined by phagocytosis and mouse inoculation. Isolates from Singapore were similar to those from Taiwan in genomic heterogeneity, prevalence of serotype, and the presence of magA and rmpA. The most common serotype was K1 (34/73; 46.6%), followed by K2 (15/73; 20.5%). magA was restricted to serotype K1. All K1 or K2 isolates and 66.7% (16/24) of isolates that were neither serotype K1 nor serotype K2 (non-K1/K2) carried rmpA. Serotype K1 or K2 isolates demonstrated significantly more phagocytic resistance and virulence than did rmpA-positive and -negative groups of non-K1/K2 isolates. In the non-K1/K2 group, the virulence profiles of rmpA-positive strains from Taiwan and Singapore were different by phagocytosis assay and in the mouse model, indicating that factors other than rmpA contributed to virulence. The characteristics of K. pneumoniae liver abscess in Singapore and Taiwan are similar. Capsular serotype K1 or K2 plays a more important role than magA and rmpA in determining virulence in K. pneumoniae liver abscess.